ARG70329
Mouse CD28 recombinant protein (ECD) (Fc-His-tagged, C-ter)
Mouse CD28 recombinant protein (ECD) (Fc-His-tagged, C-ter) for SDS-PAGE,Binding Activity and Mouse
Overview
| Product Description | HEK293 expressed, Fc-His-tagged (C-ter) Mouse CD28 recombinant protein (ECD). |
|---|---|
| Tested Reactivity | Ms |
| Tested Application | Binding, SDS-PAGE |
| Target Name | CD28 (ECD) |
| Species | Mouse |
| A.A. Sequence | Asn20 - Lys149 of Mouse CD28 (NP_031668.3) with an Fc-6X His tag at the C-terminus. |
| Expression System | HEK293 |
| Alternate Names | Tp44; TP44; T-cell-specific surface glycoprotein CD28; CD antigen CD28 |
Application Instructions
| Application Note | Binding activity test: Measured by its binding ability in a functional ELISA. Immobilized Recombinant Mouse CD86 at 5µg/ml (100 µl/well) can bind Recombinant Mouse CD28 with a linear range of 1.5-6 µg/ml. |
|---|
Properties
| Form | Powder |
|---|---|
| Purification Note | 0.22 µm filter sterilized. Endotoxin level is less than 0.1 EU/µg of the protein, as determined by the LAL test. |
| Purity | > 97% (by SDS-PAGE) |
| Buffer | PBS (pH 7.4) |
| Reconstitution | Reconstitute to a concentration of 0.1 - 0.5 mg/ml in sterile distilled water. |
| Storage Instruction | Lyophilized protein should be stored at -20°C or -80°C. After reconstitution, aliquot and store at -20°C for up to one month, at 2-8°C for up to one week. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P31041 Mouse T-cell-specific surface glycoprotein CD28 |
|---|---|
| Gene Symbol | CD28 |
| Gene Full Name | CD28 molecule |
| Background | The protein encoded by this gene is essential for T-cell proliferation and survival, cytokine production, and T-helper type-2 development. Several alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2011] |
| Function | Involved in T-cell activation, the induction of cell proliferation and cytokine production and promotion of T-cell survival. Enhances the production of IL4 and IL10 in T-cells in conjunction with TCR/CD3 ligation and CD40L costimulation (PubMed:8617933). Isoform 3 enhances CD40L-mediated activation of NF-kappa-B and kinases MAPK8 and PAK2 in T-cells (PubMed:15067037). [UniProt] |
| Cellular Localization | Membrane; Single-pass type I membrane protein. Isoform 3: Cell surface. [UniProt] |
| Calculated MW | 25 kDa |
| PTM | CD40LG induces tyrosine phosphorylation of isoform 3. [UniProt] |
