ARG70329

Mouse CD28 recombinant protein (ECD) (Fc-His-tagged, C-ter)

Mouse CD28 recombinant protein (ECD) (Fc-His-tagged, C-ter) for SDS-PAGE,Binding Activity and Mouse

Overview

Product Description HEK293 expressed, Fc-His-tagged (C-ter) Mouse CD28 recombinant protein (ECD).
Tested Reactivity Ms
Tested Application Binding, SDS-PAGE
Target Name CD28 (ECD)
Species Mouse
A.A. Sequence Asn20 - Lys149 of Mouse CD28 (NP_031668.3) with an Fc-6X His tag at the C-terminus.
Expression System HEK293
Alternate Names Tp44; TP44; T-cell-specific surface glycoprotein CD28; CD antigen CD28

Application Instructions

Application Note Binding activity test: Measured by its binding ability in a functional ELISA. Immobilized Recombinant Mouse CD86 at 5µg/ml (100 µl/well) can bind Recombinant Mouse CD28 with a linear range of 1.5-6 µg/ml.

Properties

Form Powder
Purification Note 0.22 µm filter sterilized. Endotoxin level is less than 0.1 EU/µg of the protein, as determined by the LAL test.
Purity > 97% (by SDS-PAGE)
Buffer PBS (pH 7.4)
Reconstitution Reconstitute to a concentration of 0.1 - 0.5 mg/ml in sterile distilled water.
Storage Instruction Lyophilized protein should be stored at -20°C or -80°C. After reconstitution, aliquot and store at -20°C for up to one month, at 2-8°C for up to one week. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 12487 Mouse CD28

Swiss-port # P31041 Mouse T-cell-specific surface glycoprotein CD28

Gene Symbol CD28
Gene Full Name CD28 molecule
Background The protein encoded by this gene is essential for T-cell proliferation and survival, cytokine production, and T-helper type-2 development. Several alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2011]
Function Involved in T-cell activation, the induction of cell proliferation and cytokine production and promotion of T-cell survival. Enhances the production of IL4 and IL10 in T-cells in conjunction with TCR/CD3 ligation and CD40L costimulation (PubMed:8617933). Isoform 3 enhances CD40L-mediated activation of NF-kappa-B and kinases MAPK8 and PAK2 in T-cells (PubMed:15067037). [UniProt]
Cellular Localization Membrane; Single-pass type I membrane protein. Isoform 3: Cell surface. [UniProt]
Calculated MW 25 kDa
PTM CD40LG induces tyrosine phosphorylation of isoform 3. [UniProt]