ARG81770
Mouse CD31 ELISA Kit
Mouse CD31 ELISA Kit for ELISA and Mouse
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG81770-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG81770-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG81770-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG81770-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81770-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81770-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81770-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81770-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG81770-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG81770-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG81770-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG81770 Mouse CD31 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Mouse CD31 in serum, plasma (heparin, EDTA) and cell culture supernatants. |
|---|---|
| Tested Reactivity | Ms |
| Tested Application | ELISA |
| Specificity | There is no detectable cross-reactivity with other relevant proteins. |
| Target Name | CD31 |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 78 pg/ml |
| Sample Type | Serum, plasma (heparin, EDTA) and cell culture supernatants. |
| Standard Range | 156 - 10000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 6.8%; Inter-Assay CV: 7.6% |
| Alternate Names | EndoCAM; CD31/EndoCAM; PECAM-1; CD31; PECA1; CD antigen CD31; GPIIA'; endoCAM; Platelet endothelial cell adhesion molecule |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q08481 Mouse Platelet endothelial cell adhesion molecule |
|---|---|
| Gene Symbol | PECAM1 |
| Gene Full Name | platelet/endothelial cell adhesion molecule 1 |
| Background | CD31 protein is found on the surface of platelets, monocytes, neutrophils, and some types of T-cells, and makes up a large portion of endothelial cell intercellular junctions. The encoded protein is a member of the immunoglobulin superfamily and is likely involved in leukocyte migration, angiogenesis, and integrin activation. [provided by RefSeq, May 2010] |
| Function | CD31 is a cell adhesion molecule which is required for leukocyte transendothelial migration (TEM) under most inflammatory conditions (PubMed:19342684, PubMed:17580308). Tyr-690 plays a critical role in TEM and is required for efficient trafficking of PECAM1 to and from the lateral border recycling compartment (LBRC) and is also essential for the LBRC membrane to be targeted around migrating leukocytes (PubMed:19342684). Trans-homophilic interaction may play a role in endothelial cell-cell adhesion via cell junctions (PubMed:27958302). Heterophilic interaction with CD177 plays a role in transendothelial migration of neutrophils (PubMed:17580308). Homophilic ligation of PECAM1 prevents macrophage-mediated phagocytosis of neighboring viable leukocytes by transmitting a detachment signal (PubMed:12110892). Promotes macrophage-mediated phagocytosis of apoptotic leukocytes by tethering them to the phagocytic cells; PECAM1-mediated detachment signal appears to be disabled in apoptotic leukocytes (PubMed:12110892). Modulates bradykinin receptor BDKRB2 activation (PubMed:18672896). Regulates bradykinin- and hyperosmotic shock-induced ERK1/2 activation in endothelial cells (PubMed:18672896). Induces susceptibility to atherosclerosis. Isoform Delta15: Does not protect against apoptosis. [UniProt] |
| Highlight | Related products: CD31 antibodies; CD31 ELISA Kits; CD31 Duos / Panels; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| PTM | Phosphorylated on Ser and Tyr residues after cellular activation. Phosphorylated on tyrosine residues by FER and FES in response to FCER1 activation (By similarity). In endothelial cells Fyn mediates mechanical-force (stretch or pull) induced tyrosine phosphorylation. Palmitoylation by ZDHHC21 is necessary for cell surface expression in endothelial cells and enrichment in membrane rafts. [UniProt] |
| Title | Download Link |
|---|---|
| ARG81770 Mouse CD31 ELISA Kit User manual |
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