ARG81770

Mouse CD31 ELISA Kit

Mouse CD31 ELISA Kit for ELISA and Mouse

Component

Cat No Component Name Package Temp
ARG81770-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG81770-002 Standard 2 X 10 ng/vial 4°C
ARG81770-003 Standard/Sample diluent 30 ml (Ready to use) 4°C
ARG81770-004 Antibody conjugate concentrate (100X) 1 vial (100 µl) 4°C
ARG81770-005 Antibody diluent buffer 12 ml (Ready to use) 4°C
ARG81770-006 HRP-Streptavidin concentrate (100X) 1 vial (100 µl) 4°C
ARG81770-007 HRP-Streptavidin diluent buffer 12 ml (Ready to use) 4°C
ARG81770-008 25X Wash buffer 20 ml 4°C
ARG81770-009 TMB substrate 10 ml (Ready to use) 4°C (Protect from light)
ARG81770-010 STOP solution 10 ml (Ready to use) 4°C
ARG81770-011 Plate sealer 4 strips Room temperature

Overview

Product Description ARG81770 Mouse CD31 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Mouse CD31 in serum, plasma (heparin, EDTA) and cell culture supernatants.
Tested Reactivity Ms
Tested Application ELISA
Specificity There is no detectable cross-reactivity with other relevant proteins.
Target Name CD31
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm.
Sensitivity 78 pg/ml
Sample Type Serum, plasma (heparin, EDTA) and cell culture supernatants.
Standard Range 156 - 10000 pg/ml
Sample Volume 100 µl
Precision Intra-Assay CV: 6.8%; Inter-Assay CV: 7.6%
Alternate Names EndoCAM; CD31/EndoCAM; PECAM-1; CD31; PECA1; CD antigen CD31; GPIIA'; endoCAM; Platelet endothelial cell adhesion molecule

Application Instructions

Assay Time ~ 5 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 18613 Mouse PECAM1

Swiss-port # Q08481 Mouse Platelet endothelial cell adhesion molecule

Gene Symbol PECAM1
Gene Full Name platelet/endothelial cell adhesion molecule 1
Background CD31 protein is found on the surface of platelets, monocytes, neutrophils, and some types of T-cells, and makes up a large portion of endothelial cell intercellular junctions. The encoded protein is a member of the immunoglobulin superfamily and is likely involved in leukocyte migration, angiogenesis, and integrin activation. [provided by RefSeq, May 2010]
Function CD31 is a cell adhesion molecule which is required for leukocyte transendothelial migration (TEM) under most inflammatory conditions (PubMed:19342684, PubMed:17580308). Tyr-690 plays a critical role in TEM and is required for efficient trafficking of PECAM1 to and from the lateral border recycling compartment (LBRC) and is also essential for the LBRC membrane to be targeted around migrating leukocytes (PubMed:19342684). Trans-homophilic interaction may play a role in endothelial cell-cell adhesion via cell junctions (PubMed:27958302). Heterophilic interaction with CD177 plays a role in transendothelial migration of neutrophils (PubMed:17580308). Homophilic ligation of PECAM1 prevents macrophage-mediated phagocytosis of neighboring viable leukocytes by transmitting a detachment signal (PubMed:12110892). Promotes macrophage-mediated phagocytosis of apoptotic leukocytes by tethering them to the phagocytic cells; PECAM1-mediated detachment signal appears to be disabled in apoptotic leukocytes (PubMed:12110892). Modulates bradykinin receptor BDKRB2 activation (PubMed:18672896). Regulates bradykinin- and hyperosmotic shock-induced ERK1/2 activation in endothelial cells (PubMed:18672896). Induces susceptibility to atherosclerosis.

Isoform Delta15: Does not protect against apoptosis. [UniProt]
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CD31 antibodies; CD31 ELISA Kits; CD31 Duos / Panels;
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PTM Phosphorylated on Ser and Tyr residues after cellular activation. Phosphorylated on tyrosine residues by FER and FES in response to FCER1 activation (By similarity). In endothelial cells Fyn mediates mechanical-force (stretch or pull) induced tyrosine phosphorylation.

Palmitoylation by ZDHHC21 is necessary for cell surface expression in endothelial cells and enrichment in membrane rafts. [UniProt]
Title Download Link
ARG81770 Mouse CD31 ELISA Kit User manual download icon Download