ARG82432

Mouse CD55 ELISA Kit

Mouse CD55 ELISA Kit for ELISA and Mouse

Component

Cat No Component Name Package Temp
ARG82432-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG82432-002 Standard 2 X 10 ng/vial 4°C
ARG82432-003 Standard/Sample diluent 30 ml (Ready to use) 4°C
ARG82432-004 Antibody conjugate concentrate (100X) 1 vial (100 µl) 4°C
ARG82432-005 Antibody diluent buffer 12 ml (Ready to use) 4°C
ARG82432-006 HRP-Streptavidin concentrate (100X) 1 vial (100 µl) 4°C
ARG82432-007 HRP-Streptavidin diluent buffer 12 ml (Ready to use) 4°C
ARG82432-008 25X Wash buffer 20 ml 4°C
ARG82432-009 TMB substrate 10 ml (Ready to use) 4°C (Protect from light)
ARG82432-010 STOP solution 10 ml (Ready to use) 4°C
ARG82432-011 Plate sealer 4 strips Room temperature

Overview

Product Description ARG82432 Mouse CD55 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Mouse CD55 in serum, plasma (EDTA, heparin) and cell culture supernatants.
Tested Reactivity Ms
Tested Application ELISA
Target Name CD55
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm.
Sensitivity 78 pg/ml
Sample Type Serum, plasma (EDTA, heparin) and cell culture supernatants.
Standard Range 156 - 10000 pg/ml
Sample Volume 100 µl
Precision Intra-Assay CV: 4.2%
Inter-Assay CV: 6.8%
Alternate Names DAF; CD antigen CD55; CROM; Complement decay-accelerating factor; CR; TC

Application Instructions

Assay Time ~ 5 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 13136 Mouse CD55

Swiss-port # Q61475 Mouse Complement decay-accelerating factor, GPI-anchored

Gene Symbol CD55
Gene Full Name CD55 molecule, decay accelerating factor for complement (Cromer blood group)
Background This gene encodes a glycoprotein involved in the regulation of the complement cascade. Binding of the encoded protein to complement proteins accelerates their decay, thereby disrupting the cascade and preventing damage to host cells. Antigens present on this protein constitute the Cromer blood group system (CROM). Alternative splicing results in multiple transcript variants. The predominant transcript variant encodes a membrane-bound protein, but alternatively spliced transcripts may produce soluble proteins. [provided by RefSeq, Jul 2014]
Function This protein recognizes C4b and C3b fragments that condense with cell-surface hydroxyl or amino groups when nascent C4b and C3b are locally generated during C4 and c3 activation. Interaction of daf with cell-associated C4b and C3b polypeptides interferes with their ability to catalyze the conversion of C2 and factor B to enzymatically active C2a and Bb and thereby prevents the formation of C4b2a and C3bBb, the amplification convertases of the complement cascade. [UniProt]
Cellular Localization Isoform 1: Cell membrane; Single-pass type I membrane protein. Isoform 2: Cell membrane; Lipid-anchor, GPI-anchor. Isoform 3: Secreted. Isoform 4: Secreted. Isoform 5: Secreted. Isoform 6: Cell membrane; Lipid-anchor, GPI-anchor. Isoform 7: Cell membrane; Lipid-anchor, GPI-anchor. [UniProt]
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CD55 antibodies; CD55 ELISA Kits;
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PTM The Ser/Thr-rich domain is heavily O-glycosylated. [UniProt]
Title Download Link
ARG82432 Mouse CD55 ELISA Kit User manual download icon Download