ARG81669

Mouse CD62E / E-Selectin ELISA Kit

Mouse CD62E / E-Selectin ELISA Kit for ELISA and Mouse

Component

Cat No Component Name Package Temp
ARG81669-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG81669-002 Standard 2 X 10 ng/vial 4°C
ARG81669-003 Standard/Sample diluent 30 ml (Ready to use) 4°C
ARG81669-004 Antibody conjugate concentrate (100X) 1 vial (100 µl) 4°C
ARG81669-005 Antibody diluent buffer 12 ml (Ready to use) 4°C
ARG81669-006 HRP-Streptavidin concentrate (100X) 1 vial (100 µl) 4°C
ARG81669-007 HRP-Streptavidin diluent buffer 12 ml (Ready to use) 4°C
ARG81669-008 25X Wash buffer 20 ml 4°C
ARG81669-009 TMB substrate 10 ml (Ready to use) 4°C (Protect from light)
ARG81669-010 STOP solution 10 ml (Ready to use) 4°C
ARG81669-011 Plate sealer 4 strips Room temperature

Overview

Product Description ARG81669 Mouse CD62E / E-Selectin ELISA Kit is an Enzyme Immunoassay kit for the quantification of Mouse soluble CD62E / E-Selectin in serum, plasma (heparin, citrate) and cell culture supernatants.
Tested Reactivity Ms
Tested Application ELISA
Specificity There is no detectable cross-reactivity with other relevant proteins.
Target Name CD62E / E-Selectin
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm.
Sensitivity 78 pg/ml
Sample Type Serum, plasma (heparin, citrate) and cell culture supernatants.
Standard Range 156 - 10000 pg/ml
Sample Volume 100 µl
Precision Intra-Assay CV: 5.1%; Inter-Assay CV: 6.3%
Alternate Names E-selectin; CD62E; ELAM-1; ELAM1; CD antigen CD62E; ELAM; Endothelial leukocyte adhesion molecule 1; Leukocyte-endothelial cell adhesion molecule 2; ESEL; LECAM2; CD62 antigen-like family member E

Application Instructions

Assay Time ~ 5 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 20339 Mouse SELE

Swiss-port # Q00690 Mouse E-selectin

Gene Symbol SELE
Gene Full Name selectin E
Background The protein encoded by this gene is found in cytokine-stimulated endothelial cells and is thought to be responsible for the accumulation of blood leukocytes at sites of inflammation by mediating the adhesion of cells to the vascular lining. It exhibits structural features such as the presence of lectin- and EGF-like domains followed by short consensus repeat (SCR) domains that contain 6 conserved cysteine residues. These proteins are part of the selectin family of cell adhesion molecules. Adhesion molecules participate in the interaction between leukocytes and the endothelium and appear to be involved in the pathogenesis of atherosclerosis. [provided by RefSeq, Jul 2008]
Function Cell-surface glycoprotein having a role in immunoadhesion. Mediates in the adhesion of blood neutrophils in cytokine-activated endothelium through interaction with PSGL1/SELPLG. May have a role in capillary morphogenesis. [UniProt]
Highlight Related products:
CD62E antibodies; CD62E ELISA Kits;
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Simplify the ELISA analysis by GainData
Title Download Link
ARG81669 Mouse sCD62E / sE-Selectin ELISA Kit User manual download icon Download