ARG81671

Mouse CD62P / P-Selectin ELISA Kit

Mouse CD62P / P-Selectin ELISA Kit for ELISA and Mouse

Component

Cat No Component Name Package Temp
ARG81671-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG81671-002 Standard 2 X 20 ng/vial 4°C
ARG81671-003 Standard/Sample diluent 30 ml (Ready to use) 4°C
ARG81671-004 Antibody conjugate concentrate (100X) 1 vial (100 µl) 4°C
ARG81671-005 Antibody diluent buffer 12 ml (Ready to use) 4°C
ARG81671-006 HRP-Streptavidin concentrate (100X) 1 vial (100 µl) 4°C
ARG81671-007 HRP-Streptavidin diluent buffer 12 ml (Ready to use) 4°C
ARG81671-008 25X Wash buffer 20 ml 4°C
ARG81671-009 TMB substrate 10 ml (Ready to use) 4°C (Protect from light)
ARG81671-010 STOP solution 10 ml (Ready to use) 4°C
ARG81671-011 Plate sealer 4 strips Room temperature

Overview

Product Description ARG81671 Mouse CD62P / P-Selectin ELISA Kit is an Enzyme Immunoassay kit for the quantification of Mouse CD62P / P-Selectin in serum, plasma (citrate) and cell culture supernatants.
Tested Reactivity Ms
Tested Application ELISA
Specificity There is no detectable cross-reactivity with other relevant proteins.
Target Name CD62P / P-Selectin
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm.
Sensitivity 156 pg/ml
Sample Type Serum, plasma (citrate) and cell culture supernatants.
Standard Range 312 - 20000 pg/ml
Sample Volume 100 µl
Precision Intra-Assay CV: 5.3%; Inter-Assay CV: 5.9%
Alternate Names PADGEM; CD62; Platelet activation dependent granule-external membrane protein; CD62 antigen-like family member P; GMP140; PSEL; Granule membrane protein 140; CD62P; CD antigen CD62P; Leukocyte-endothelial cell adhesion molecule 3; GRMP; GMP-140; P-selectin; LECAM3

Application Instructions

Assay Time ~ 5 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 20344 Mouse SELP

Swiss-port # Q01102 Mouse P-selectin

Gene Symbol SELP
Gene Full Name selectin P (granule membrane protein 140kDa, antigen CD62)
Background This gene encodes a 140 kDa protein that is stored in the alpha-granules of platelets and Weibel-Palade bodies of endothelial cells. This protein redistributes to the plasma membrane during platelet activation and degranulation and mediates the interaction of activated endothelial cells or platelets with leukocytes. The membrane protein is a calcium-dependent receptor that binds to sialylated forms of Lewis blood group carbohydrate antigens on neutrophils and monocytes. Alternative splice variants may occur but are not well documented. [provided by RefSeq, Jul 2008]
Function Ca(2+)-dependent receptor for myeloid cells that binds to carbohydrates on neutrophils and monocytes. Mediates the interaction of activated endothelial cells or platelets with leukocytes. The ligand recognized is sialyl-Lewis X. Mediates rapid rolling of leukocyte rolling over vascular surfaces during the initial steps in inflammation through interaction with PSGL1. [UniProt]
Highlight Related products:
CD62 antibodies; CD62 ELISA Kits;
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Simplify the ELISA analysis by GainData
Title Download Link
ARG81671 Mouse CD62P / P-Selectin ELISA Kit User's manual download icon Download