ARG82696

Mouse CD70 ELISA Kit

Mouse CD70 ELISA Kit for ELISA and Mouse

Component

Cat No Component Name Package Temp
ARG82696-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG82696-002 Standard 2 X 10 ng/vial 4°C
ARG82696-003 Standard/Sample diluent 30 ml (Ready to use) 4°C
ARG82696-004 Antibody conjugate concentrate (100X) 1 vial (100 µl) 4°C
ARG82696-005 Antibody diluent buffer 12 ml (Ready to use) 4°C
ARG82696-006 HRP-Streptavidin concentrate (100X) 1 vial (100 µl) 4°C
ARG82696-007 HRP-Streptavidin diluent buffer 12 ml (Ready to use) 4°C
ARG82696-008 25X Wash buffer 20 ml 4°C
ARG82696-009 TMB substrate 10 ml (Ready to use) 4°C (Protect from light)
ARG82696-010 STOP solution 10 ml (Ready to use) 4°C
ARG82696-011 Plate sealer 4 strips Room temperature

Overview

Product Description ARG82696 Mouse CD70 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Mouse CD70 in serum, plasma (EDTA, heparin) and cell culture supernatants.
Tested Reactivity Ms
Tested Application ELISA
Target Name CD70
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm.
Sensitivity 16 pg/ml
Sample Type Serum, plasma (EDTA, heparin) and cell culture supernatants.
Standard Range 31.2 - 2000 pg/ml
Sample Volume 100 µl
Precision Intra-Assay CV: 6.6%
Inter-Assay CV: 7.4%
Alternate Names CD27 ligand; CD70 antigen; Tumor necrosis factor ligand superfamily member 7; CD antigen CD70; CD27L; CD27-L; TNFSF7; CD27LG

Application Instructions

Assay Time ~ 5 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 21948 Mouse CD70

Swiss-port # O55237 Mouse CD70 antigen

Gene Symbol CD70
Gene Full Name CD70 molecule
Background The protein encoded by this gene is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This cytokine is a ligand for TNFRSF27/CD27. It is a surface antigen on activated, but not on resting, T and B lymphocytes. It induces proliferation of costimulated T cells, enhances the generation of cytolytic T cells, and contributes to T cell activation. This cytokine is also reported to play a role in regulating B-cell activation, cytotoxic function of natural killer cells, and immunoglobulin sythesis. [provided by RefSeq, Jul 2008]
Function Cytokine that binds to CD27. Plays a role in T-cell activation. Induces the proliferation of costimulated T-cells and enhances the generation of cytolytic T-cells. [UniProt]
Cellular Localization Membrane; Single-pass type II membrane protein. [UniProt]
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CD70 antibodies; CD70 ELISA Kits;
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Title Download Link
ARG82696 Mouse CD70 ELISA kit User manual download icon Download