ARG70337

Mouse CD80 recombinant protein (ECD) (Fc-His-tagged, C-ter)

Mouse CD80 recombinant protein (ECD) (Fc-His-tagged, C-ter) for SDS-PAGE,Binding Activity and Mouse

Overview

Product Description HEK293 expressed, Fc-His-tagged (C-ter) Mouse CD80 recombinant protein (ECD).
Tested Reactivity Ms
Tested Application Binding, SDS-PAGE
Target Name CD80 (ECD)
Species Mouse
A.A. Sequence Val38 - Lys245 of Mouse CD80 (NP_033985.3) with an Fc-6X His tag at the C-terminus.
Expression System HEK293
Alternate Names B7.1; CTLA-4 counter-receptor B7.1; CD28LG; T-lymphocyte activation antigen CD80; B7-1; CD28LG1; B7; LAB7; Activation B7-1 antigen; CD antigen CD80; BB1

Application Instructions

Application Note Binding activity test: Measured by its binding ability in a functional ELISA. Immobilized Recombinant Mouse CD80 at 500ng/ml (100 µl/well) can bind Recombinant Human CTLA-4 with a linear range of 11-44 ng/ml.

Properties

Form Powder
Purification Note 0.22 µm filter sterilized. Endotoxin level is less than 0.1 EU/µg of the protein, as determined by the LAL test.
Purity > 97% (by SDS-PAGE)
Buffer PBS (pH 7.4)
Reconstitution Reconstitute to a concentration of 0.1 - 0.5 mg/ml in sterile distilled water.
Storage Instruction Lyophilized protein should be stored at -20°C or -80°C. After reconstitution, aliquot and store at -20°C for up to one month, at 2-8°C for up to one week. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 12519 Mouse CD80

Swiss-port # Q00609 Mouse T-lymphocyte activation antigen CD80

Gene Symbol CD80
Gene Full Name CD80 molecule
Background The protein encoded by this gene is a membrane receptor that is activated by the binding of CD28 or CTLA-4. The activated protein induces T-cell proliferation and cytokine production. This protein can act as a receptor for adenovirus subgroup B and may play a role in lupus neuropathy. [provided by RefSeq, Aug 2011]
Function Involved in the costimulatory signal essential for T-lymphocyte activation. T-cell proliferation and cytokine production is induced by the binding of CD28, binding to CTLA-4 has opposite effects and inhibits T-cell activation.

(Microbial infection) Acts as a receptor for adenovirus subgroup B. [UniProt]
Cellular Localization Membrane; Single-pass type I membrane protein. [UniProt]
Calculated MW 33 kDa