ARG82746

Mouse CD99 ELISA Kit

Mouse CD99 ELISA Kit for ELISA and Mouse

Component

Cat No Component Name Package Temp
ARG82746-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG82746-002 Standard 2 X 10 ng/vial 4°C
ARG82746-003 Standard/Sample diluent 30 ml (Ready to use) 4°C
ARG82746-004 Antibody conjugate concentrate (100X) 1 vial (100 µl) 4°C
ARG82746-005 Antibody diluent buffer 12 ml (Ready to use) 4°C
ARG82746-006 HRP-Streptavidin concentrate (100X) 1 vial (100 µl) 4°C
ARG82746-007 HRP-Streptavidin diluent buffer 12 ml (Ready to use) 4°C
ARG82746-008 25X Wash buffer 20 ml 4°C
ARG82746-009 TMB substrate 10 ml (Ready to use) 4°C (Protect from light)
ARG82746-010 STOP solution 10 ml (Ready to use) 4°C
ARG82746-011 Plate sealer 4 strips Room temperature

Overview

Product Description ARG82746 Mouse CD99 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Mouse CD99 in serum, plasma (EDTA, heparin, citrate) and cell culture supernatants.
Tested Reactivity Ms
Tested Application ELISA
Target Name CD99
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm.
Sensitivity 63 pg/ml
Sample Type Serum, plasma (EDTA, heparin, citrate) and cell culture supernatants.
Standard Range 125 - 8000 pg/ml
Sample Volume 100 µl
Precision Intra-Assay CV: 4.3%
Inter-Assay CV: 5.9%
Alternate Names 12E7; CD99 antigen; MIC2X; MIC2Y; CD antigen CD99; MSK5X; Protein MIC2; MIC2; T-cell surface glycoprotein E2; HBA71; E2 antigen

Application Instructions

Assay Time ~ 5 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 673094 Mouse CD99

Swiss-port # Q8VCN6 Mouse CD99 antigen

Gene Symbol CD99
Gene Full Name CD99 molecule
Background The protein encoded by this gene is a cell surface glycoprotein involved in leukocyte migration, T-cell adhesion, ganglioside GM1 and transmembrane protein transport, and T-cell death by a caspase-independent pathway. In addition, the encoded protein may have the ability to rearrange the actin cytoskeleton and may also act as an oncosuppressor in osteosarcoma. Cyclophilin A binds to CD99 and may act as a signaling regulator of CD99. This gene is found in the pseudoautosomal region of chromosomes X and Y and escapes X-chromosome inactivation. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2013]
Function Involved in T-cell adhesion processes and in spontaneous rosette formation with erythrocytes. Plays a role in a late step of leukocyte extravasation helping leukocytes to overcome the endothelial basement membrane. Acts at the same site as, but independently of, PECAM1. Involved in T-cell adhesion processes (By similarity). [UniProt]
Cellular Localization Membrane; Single-pass type I membrane protein. [UniProt]
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PTM Extensively O-glycosylated. [UniProt]
Title Download Link
ARG82746 Mouse CD99 ELISA kit User manual download icon Download