ARG70199
Mouse CNTF recombinant protein (Active) (His-tagged, C-ter)
Mouse CNTF recombinant protein (Active) (His-tagged, C-ter) for SDS-PAGE
Overview
| Product Description | E. coli expressed, His-tagged (C-ter) Active Mouse CNTF recombinant protein |
|---|---|
| Tested Application | SDS-PAGE |
| Target Name | CNTF |
| Species | Mouse |
| A.A. Sequence | MAFAE QSPLT LHRRD LCSRS IWLAR KIRSD LTALM ESYVK HQGLN KNISL DSVDG VPVAS TDRWS EMTEA ERLQE NLQAY RTFQG MLTKL LEDQR VHFTP TEGDF HQAIH TLTLQ VSAFA YQLEE LMALL EQKVP EKEAD GMPVT IGDGG LFEKK LWGLK VLQEL SQWTV RSIHD LRVIS SHHMG ISAHE SHYGA KQM with polyhistidine tag at the C-terminus. |
| Expression System | E. coli |
| Activity | Active |
| Activity Note | Determined by its ability to induce proliferation in TF-1 cells. The ED50 for this effect is < 10 ng/ml. The specific activity of recombinant mouse CNTF is > 1 x 10^5 IU/mg. |
| Alternate Names | HCNTF; CNTF; Ciliary neurotrophic factor |
Properties
| Form | Powder |
|---|---|
| Purification | Ni-NTA chromatography. |
| Purification Note | Endotoxin level is less than 0.1 EU/µg of the protein, as determined by the LAL test. |
| Purity | > 98% (by SDS-PAGE) |
| Buffer | PBS (pH 7.4) |
| Reconstitution | It is recommended to reconstitute the lyophilized protein in sterile water to a concentration not less than 100 µg/ml and incubate the stock solution for at least 20 min at room temperature to make sure the protein is dissolved completely. |
| Storage Instruction | Lyophilized protein should be stored at -20°C. After reconstitution, aliquot and store at -20°C or -80°C for up to one month. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Gene Symbol | CNTF |
|---|---|
| Gene Full Name | ciliary neurotrophic factor |
| Background | The protein encoded by this gene is a polypeptide hormone whose actions appear to be restricted to the nervous system where it promotes neurotransmitter synthesis and neurite outgrowth in certain neuronal populations. The protein is a potent survival factor for neurons and oligodendrocytes and may be relevant in reducing tissue destruction during inflammatory attacks. A mutation in this gene, which results in aberrant splicing, leads to ciliary neurotrophic factor deficiency, but this phenotype is not causally related to neurologic disease. A read-through transcript variant composed of the upstream ZFP91 gene and CNTF sequence has been identified, but it is thought to be non-coding. Read-through transcription of ZFP91 and CNTF has also been observed in mouse. [provided by RefSeq, Oct 2010] |
| Function | CNTF is a survival factor for various neuronal cell types. Seems to prevent the degeneration of motor axons after axotomy. [UniProt] |
| Cellular Localization | Cytoplasm. [UniProt] |
