ARG81646

Mouse CX3CL1 ELISA Kit

Mouse CX3CL1 ELISA Kit for ELISA and Mouse

Component

Cat No Component Name Package Temp
ARG81646-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG81646-002 Standard 2 X 20 ng/vial 4°C
ARG81646-003 Standard/Sample diluent 30 ml (Ready to use) 4°C
ARG81646-004 Antibody conjugate concentrate (100X) 1 vial (100 µl) 4°C
ARG81646-005 Antibody diluent buffer 12 ml (Ready to use) 4°C
ARG81646-006 HRP-Streptavidin concentrate (100X) 1 vial (100 µl) 4°C
ARG81646-007 HRP-Streptavidin diluent buffer 12 ml (Ready to use) 4°C
ARG81646-008 25X Wash buffer 20 ml 4°C
ARG81646-009 TMB substrate 10 ml (Ready to use) 4°C (Protect from light)
ARG81646-010 STOP solution 10 ml (Ready to use) 4°C
ARG81646-011 Plate sealer 4 strips Room temperature

Overview

Product Description ARG81646 Mouse CX3CL1 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Mouse CX3CL1 in serum, plasma (heparin, EDTA) and cell culture supernatants.
Tested Reactivity Ms
Tested Application ELISA
Specificity There is no detectable cross-reactivity with Eotaxin, MCP1, MCP2, CCL21 and MARC.
Target Name CX3CL1
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm.
Sensitivity 156 pg/ml
Sample Type Serum, plasma (heparin, EDTA) and cell culture supernatants.
Standard Range 312 - 20000 pg/ml
Sample Volume 100 µl
Precision Intra-Assay CV: 5.5%; Inter-Assay CV: 6.9%
Alternate Names neurotactin; CXC3; C3Xkine; NTN; NTT; C-X3-C motif chemokine 1; CXC3C; Neurotactin; ABCD-3; SCYD1; Fractalkine; Small-inducible cytokine D1; CX3C membrane-anchored chemokine; fractalkine

Application Instructions

Assay Time ~ 5 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 20312 Mouse CX3CL1

Swiss-port # O35188 Mouse Fractalkine

Gene Symbol CX3CL1
Gene Full Name chemokine (C-X3-C motif) ligand 1
Function The soluble form is chemotactic for T-cells and monocytes, but not for neutrophils. The membrane-bound form promotes adhesion of those leukocytes to endothelial cells. May play a role in regulating leukocyte adhesion and migration processes at the endothelium. Binds to CX3CR1. [UniProt]
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PTM A soluble short 95 kDa form may be released by proteolytic cleavage from the long membrane-anchored form.

O-glycosylated with core 1 or possibly core 8 glycans. [UniProt]
Title Download Link
ARG81646 Mouse CX3CL1 ELISA Kit User's manual download icon Download