ARG82549

Mouse Cadherin 12 ELISA Kit

Mouse Cadherin 12 ELISA Kit for ELISA and Mouse

Component

Cat No Component Name Package Temp
ARG82549-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG82549-002 Standard 2 X 10 ng/vial 4°C
ARG82549-003 Standard/Sample diluent 30 ml (Ready to use) 4°C
ARG82549-004 Antibody conjugate concentrate (100X) 1 vial (100 µl) 4°C
ARG82549-005 Antibody diluent buffer 12 ml (Ready to use) 4°C
ARG82549-006 HRP-Streptavidin concentrate (100X) 1 vial (100 µl) 4°C
ARG82549-007 HRP-Streptavidin diluent buffer 12 ml (Ready to use) 4°C
ARG82549-008 25X Wash buffer 20 ml 4°C
ARG82549-009 TMB substrate 10 ml (Ready to use) 4°C (Protect from light)
ARG82549-010 STOP solution 10 ml (Ready to use) 4°C
ARG82549-011 Plate sealer 4 strips Room temperature

Overview

Product Description ARG82549 Mouse Cadherin 12 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Mouse Cadherin 12 in serum, plasma (EDTA, heparin) and cell culture supernatants.
Tested Reactivity Ms
Tested Application ELISA
Target Name Cadherin 12
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm.
Sensitivity 78 pg/ml
Sample Type Serum, plasma (EDTA, heparin) and cell culture supernatants.
Standard Range 156 - 10000 pg/ml
Sample Volume 100 µl
Precision Intra-Assay CV: 4.2%
Inter-Assay CV: 7.1%
Alternate Names Cadherin-12; Brain cadherin; CDHB; Neural type cadherin 2; BR-cadherin; N-cadherin 2

Application Instructions

Assay Time ~ 5 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 215654 Mouse CDH12

Swiss-port # Q5RJH3 Mouse Cadherin-12

Gene Symbol CDH12
Gene Full Name cadherin 12, type 2 (N-cadherin 2)
Background This gene encodes a type II classical cadherin from the cadherin superfamily of integral membrane proteins that mediate calcium-dependent cell-cell adhesion. Mature cadherin proteins are composed of a large N-terminal extracellular domain, a single membrane-spanning domain, and a small, highly conserved C-terminal cytoplasmic domain. Type II (atypical) cadherins are defined based on their lack of a HAV cell adhesion recognition sequence specific to type I cadherins. This particular cadherin appears to be expressed specifically in the brain and its temporal pattern of expression would be consistent with a role during a critical period of neuronal development, perhaps specifically during synaptogenesis. [provided by RefSeq, Jul 2008]
Function Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. [UniProt]
Cellular Localization Cell membrane; Single-pass type I membrane protein. [UniProt]
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Cadherin antibodies; Cadherin ELISA Kits; Cadherin Duos / Panels;
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Title Download Link
ARG82549 Mouse Cadherin 12 ELISA Kit User manual download icon Download