ARG81800

Mouse Complement C5 (C5a) ELISA Kit

Mouse Complement C5 (C5a) ELISA Kit for ELISA and Mouse

Component

Cat No Component Name Package Temp
ARG81800-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG81800-002 Standard 2 X 10 ng/vial 4°C
ARG81800-003 Standard/Sample diluent 30 ml (Ready to use) 4°C
ARG81800-004 Antibody conjugate concentrate (100X) 1 vial (100 µl) 4°C
ARG81800-005 Antibody diluent buffer 12 ml (Ready to use) 4°C
ARG81800-006 HRP-Streptavidin concentrate (100X) 1 vial (100 µl) 4°C
ARG81800-007 HRP-Streptavidin diluent buffer 12 ml (Ready to use) 4°C
ARG81800-008 25X Wash buffer 20 ml 4°C
ARG81800-009 TMB substrate 10 ml (Ready to use) 4°C (Protect from light)
ARG81800-010 STOP solution 10 ml (Ready to use) 4°C
ARG81800-011 Plate sealer 4 strips Room temperature

Overview

Product Description ARG81800 Mouse Complement C5 (C5a) ELISA Kit is an Enzyme Immunoassay kit for the quantification of Mouse Complement C5 (C5a) in serum, plasma (heparin, EDTA) and cell culture supernatants.
Tested Reactivity Ms
Tested Application ELISA
Specificity There is no detectable cross-reactivity with other relevant proteins.
Target Name C5a / Complement C5
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm.
Sensitivity 7.8 pg/ml
Sample Type Serum, plasma (heparin, EDTA) and cell culture supernatants.
Standard Range 15.6 - 1000 pg/ml
Sample Volume 100 µl
Precision Intra-Assay CV: 6.6%; Inter-Assay CV: 7.9%
Alternate Names ECLZB; CPAMD4; Complement C5; C5D; C5b; C5a; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 4

Application Instructions

Assay Time ~ 5 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Gene Symbol C5
Gene Full Name complement component 5
Background The protein encoded by this gene is the fifth component of complement, which plays an important role in inflammatory and cell killing processes. This protein is comprised of alpha and beta polypeptide chains that are linked by a disulfide bridge. An activation peptide, C5a, which is an anaphylatoxin that possesses potent spasmogenic and chemotactic activity, is derived from the alpha polypeptide via cleavage with a convertase. The C5b macromolecular cleavage product can form a complex with the C6 complement component, and this complex is the basis for formation of the membrane attack complex, which includes additional complement components. Mutations in this gene cause complement component 5 deficiency, a disease where patients show a propensity for severe recurrent infections. Defects in this gene have also been linked to a susceptibility to liver fibrosis and to rheumatoid arthritis. [provided by RefSeq, Jul 2008]
Function Activation of C5 by a C5 convertase initiates the spontaneous assembly of the late complement components, C5-C9, into the membrane attack complex. C5b has a transient binding site for C6. The C5b-C6 complex is the foundation upon which the lytic complex is assembled.

Derived from proteolytic degradation of complement C5, C5 anaphylatoxin is a mediator of local inflammatory process. Binding to the receptor C5AR1 induces a variety of responses including intracellular calcium release, contraction of smooth muscle, increased vascular permeability, and histamine release from mast cells and basophilic leukocytes. C5a is also a potent chemokine which stimulates the locomotion of polymorphonuclear leukocytes and directs their migration toward sites of inflammation. [UniProt]
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Complement component antibodies; Complement component ELISA Kits; Complement component Duos / Panels;
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