ARG81913

Mouse Complement H ELISA Kit

Mouse Complement H ELISA Kit for ELISA and Mouse

Component

Cat No Component Name Package Temp
ARG81913-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG81913-002 Standard 2 X 50 ng/vial 4°C
ARG81913-003 Standard/Sample diluent 30 ml (Ready to use) 4°C
ARG81913-004 Antibody conjugate concentrate (100X) 1 vial (100 µl) 4°C
ARG81913-005 Antibody diluent buffer 12 ml (Ready to use) 4°C
ARG81913-006 HRP-Streptavidin concentrate (100X) 1 vial (100 µl) 4°C
ARG81913-007 HRP-Streptavidin diluent buffer 12 ml (Ready to use) 4°C
ARG81913-008 25X Wash buffer 20 ml 4°C
ARG81913-009 TMB substrate 10 ml (Ready to use) 4°C (Protect from light)
ARG81913-010 STOP solution 10 ml (Ready to use) 4°C
ARG81913-011 Plate sealer 4 strips Room temperature

Overview

Product Description ARG81913 Mouse Complement H ELISA Kit is an Enzyme Immunoassay kit for the quantification of Mouse Complement H in serum, plasma (heparin, EDTA) and cell culture supernatants.
Tested Reactivity Ms
Tested Application ELISA
Specificity There is no detectable cross-reactivity with other relevant proteins.
Target Name Complement factor H
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm.
Sensitivity 0.39 ng/ml
Sample Type Serum, plasma (heparin, EDTA) and cell culture supernatants.
Standard Range 0.78 - 50 ng/ml
Sample Volume 100 µl
Precision Intra-Assay CV: 6.3%; Inter-Assay CV: 7.2%
Alternate Names Complement factor H; ARMD4; HF1; HF2; AMBP1; ARMS1; FHL1; HUS; FH; AHUS1; H factor 1; HF; CFHL3

Application Instructions

Assay Time ~ 5 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 12628 Mouse CFH

Swiss-port # P06909 Mouse Complement factor H

Gene Symbol CFH
Gene Full Name complement factor H
Background This gene is a member of the Regulator of Complement Activation (RCA) gene cluster and encodes a protein with twenty short consensus repeat (SCR) domains. This protein is secreted into the bloodstream and has an essential role in the regulation of complement activation, restricting this innate defense mechanism to microbial infections. Mutations in this gene have been associated with hemolytic-uremic syndrome (HUS) and chronic hypocomplementemic nephropathy. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Oct 2011]
Function Factor H functions as a cofactor in the inactivation of C3b by factor I and also increases the rate of dissociation of the C3bBb complex (C3 convertase) and the (C3b)NBB complex (C5 convertase) in the alternative complement pathway. [UniProt]
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Title Download Link
ARG81913 Mouse Complement H ELISA Kit User's manual download icon Download