Component

Overview

Product Description	ARG82340 Mouse DMP1 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Mouse DMP1 in serum, plasma (EDTA, heparin) and cell culture supernatants.
Tested Reactivity	Ms
Tested Application	ELISA
Target Name	DMP1
Conjugation	HRP
Conjugation Note	Substrate: TMB and read at 450 nm.
Sensitivity	78 pg/ml
Sample Type	Serum, plasma (EDTA, heparin) and cell culture supernatants.
Standard Range	156 - 10000 pg/ml
Sample Volume	100 µl
Precision	Intra-Assay CV: 6.5% Inter-Assay CV: 7.3%
Alternate Names	DMP-1; Dentin matrix protein 1; ARHP; Dentin matrix acidic phosphoprotein 1; ARHR

Application Instructions

Assay Time	~ 5 hours

Properties

Form	96 well
Storage Instruction	Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note	For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links	GeneID: 13406 Mouse DMP1 Swiss-port # O55188 Mouse Dentin matrix acidic phosphoprotein 1
Gene Symbol	DMP1
Gene Full Name	dentin matrix acidic phosphoprotein 1
Background	Dentin matrix acidic phosphoprotein is an extracellular matrix protein and a member of the small integrin binding ligand N-linked glycoprotein family. This protein, which is critical for proper mineralization of bone and dentin, is present in diverse cells of bone and tooth tissues. The protein contains a large number of acidic domains, multiple phosphorylation sites, a functional arg-gly-asp cell attachment sequence, and a DNA binding domain. In undifferentiated osteoblasts it is primarily a nuclear protein that regulates the expression of osteoblast-specific genes. During osteoblast maturation the protein becomes phosphorylated and is exported to the extracellular matrix, where it orchestrates mineralized matrix formation. Mutations in the gene are known to cause autosomal recessive hypophosphatemia, a disease that manifests as rickets and osteomalacia. The gene structure is conserved in mammals. Two transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Jul 2008]
Function	May have a dual function during osteoblast differentiation. In the nucleus of undifferentiated osteoblasts, unphosphorylated form acts as a transcriptional component for activation of osteoblast-specific genes like osteocalcin. During the osteoblast to osteocyte transition phase it is phosphorylated and exported into the extracellular matrix, where it regulates nucleation of hydroxyapatite. [UniProt]
Cellular Localization	Nucleus. Cytoplasm. Secreted, extracellular space, extracellular matrix. Note=In proliferating preosteoblasts it is nuclear, during early maturation stage is cytoplasmic and in mature osteoblast localizes in the mineralized matrix. Export from the nucleus of differentiating osteoblast is triggered by the release of calcium from intracellular stores followed by a massive influx of this pool of calcium into the nucleus. [UniProt]
Highlight	Related products: DMP1 antibodies; DMP1 ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData
PTM	Phosphorylated in the cytosol and extracellular matrix and unphosphorylated in the nucleus. Phosphorylation is necessary for nucleocytoplasmic transport and may be catalyzed by a nuclear isoform of CK2 and can be augmented by calcium. Phosphorylated (in vitro) by FAM20C in the extracellular medium at sites within the S-x-E/pS motif. [UniProt]