ARG81941

Mouse DR3 / TNFRSF25 ELISA Kit

Mouse DR3 / TNFRSF25 ELISA Kit for ELISA and Mouse

Component

Cat No Component Name Package Temp
ARG81941-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG81941-002 Standard 2 X 10 ng/vial 4°C
ARG81941-003 Standard/Sample diluent 30 ml (Ready to use) 4°C
ARG81941-004 Antibody conjugate concentrate (100X) 1 vial (100 µl) 4°C
ARG81941-005 Antibody diluent buffer 12 ml (Ready to use) 4°C
ARG81941-006 HRP-Streptavidin concentrate (100X) 1 vial (100 µl) 4°C
ARG81941-007 HRP-Streptavidin diluent buffer 12 ml (Ready to use) 4°C
ARG81941-008 25X Wash buffer 20 ml 4°C
ARG81941-009 TMB substrate 10 ml (Ready to use) 4°C (Protect from light)
ARG81941-010 STOP solution 10 ml (Ready to use) 4°C
ARG81941-011 Plate sealer 4 strips Room temperature

Overview

Product Description ARG81941 Mouse DR3 / TNFRSF25 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Mouse DR3 / TNFRSF25 in serum, plasma (heparin, EDTA) and cell culture supernatants.
Tested Reactivity Ms
Tested Application ELISA
Specificity There is no detectable cross-reactivity with other relevant proteins.
Target Name DR3 / TNFRSF25
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm.
Sensitivity 78 pg/ml
Sample Type Serum, plasma (heparin, EDTA) and cell culture supernatants.
Standard Range 156 - 10000 pg/ml
Sample Volume 100 µl
Precision Intra-Assay CV: 5.6%; Inter-Assay CV: 6.4%
Alternate Names WSL-1; Apo-3; Apoptosis-mediating receptor TRAMP; DDR3; DR3; Protein WSL; Protein WSL-1; TRAMP; Apoptosis-inducing receptor AIR; TNFRSF12; Death receptor 3; LARD; Apoptosis-mediating receptor DR3; TR3; APO-3; Lymphocyte-associated receptor of death; Tumor necrosis factor receptor superfamily member 25; WSL-LR

Application Instructions

Assay Time ~ 5 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 85030 Mouse TNFRSF25

Gene Symbol TNFRSF25
Gene Full Name tumor necrosis factor receptor superfamily, member 25
Background The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor is expressed preferentially in the tissues enriched in lymphocytes, and it may play a role in regulating lymphocyte homeostasis. This receptor has been shown to stimulate NF-kappa B activity and regulate cell apoptosis. The signal transduction of this receptor is mediated by various death domain containing adaptor proteins. Knockout studies in mice suggested the role of this gene in the removal of self-reactive T cells in the thymus. Multiple alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported, most of which are potentially secreted molecules. The alternative splicing of this gene in B and T cells encounters a programmed change upon T-cell activation, which predominantly produces full-length, membrane bound isoforms, and is thought to be involved in controlling lymphocyte proliferation induced by T-cell activation. [provided by RefSeq, Jul 2008]
Function Receptor for TNFSF12/APO3L/TWEAK. Interacts directly with the adapter TRADD. Mediates activation of NF-kappa-B and induces apoptosis. May play a role in regulating lymphocyte homeostasis. [UniProt]
Highlight Related products:
TNFRSF25 antibodies; TNFRSF25 ELISA Kits;
New ELISA data calculation tool:
Simplify the ELISA analysis by GainData
PTM Glycosylated. [UniProt]
Title Download Link
ARG81941 Mouse DR3 / TNFRSF25 ELISA Kit User's manual download icon Download