ARG81638

Mouse EGF ELISA Kit

Mouse EGF ELISA Kit for ELISA and Mouse

Component

Cat No Component Name Package Temp
ARG81638-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG81638-002 Standard 2 X 10 ng/vial 4°C
ARG81638-003 Standard/Sample diluent 30 ml (Ready to use) 4°C
ARG81638-004 Antibody conjugate concentrate (100X) 1 vial (100 µl) 4°C
ARG81638-005 Antibody diluent buffer 12 ml (Ready to use) 4°C
ARG81638-006 HRP-Streptavidin concentrate (100X) 1 vial (100 µl) 4°C
ARG81638-007 HRP-Streptavidin diluent buffer 12 ml (Ready to use) 4°C
ARG81638-008 25X Wash buffer 20 ml 4°C
ARG81638-009 TMB substrate 10 ml (Ready to use) 4°C (Protect from light)
ARG81638-010 STOP solution 10 ml (Ready to use) 4°C
ARG81638-011 Plate sealer 4 strips Room temperature

Overview

Product Description ARG81638 Mouse EGF ELISA Kit is an Enzyme Immunoassay kit for the quantification of Mouse EGF in serum, plasma (heparin, EDTA), urine and cell culture supernatants.
Tested Reactivity Ms
Tested Application ELISA
Specificity There is no detectable cross-reactivity with other relevant proteins.
Target Name EGF
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm.
Sensitivity 3.9 pg/ml
Sample Type Serum, plasma (heparin, EDTA), urine and cell culture supernatants.
Standard Range 7.8 - 500 pg/ml
Sample Volume 100 µl
Precision Intra-Assay CV: 6.9%; Inter-Assay CV: 7.8%
Alternate Names Urogastrone; Pro-epidermal growth factor; URG; HOMG4; EGF

Application Instructions

Assay Time ~ 5 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 13645 Mouse EGF

Swiss-port # P01132 Mouse Pro-epidermal growth factor

Gene Symbol EGF
Gene Full Name epidermal growth factor
Background This gene encodes a member of the epidermal growth factor superfamily. The encoded protein is synthesized as a large precursor molecule that is proteolytically cleaved to generate the 53-amino acid epidermal growth factor peptide. This protein acts a potent mitogenic factor that plays an important role in the growth, proliferation and differentiation of numerous cell types. This protein acts by binding the high affinity cell surface receptor, epidermal growth factor receptor. Defects in this gene are the cause of hypomagnesemia type 4. Dysregulation of this gene has been associated with the growth and progression of certain cancers. Alternate splicing results in multiple transcript variants.[provided by RefSeq, May 2010]
Function EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. Magnesiotropic hormone that stimulates magnesium reabsorption in the renal distal convoluted tubule via engagement of EGFR and activation of the magnesium channel TRPM6. Can induce neurite outgrowth in motoneurons of the pond snail Lymnaea stagnalis in vitro. [UniProt]
Highlight Related products:
EGF antibodies; EGF ELISA Kits; EGF Duos / Panels; EGF recombinant proteins;
New ELISA data calculation tool:
Simplify the ELISA analysis by GainData
PTM O-glycosylated with core 1-like and core 2-like glycans. It is uncertain if Ser-954 or Thr-955 is O-glycosylated. The modification here shows glycan heterogeneity: HexHexNAc (major) and Hex2HexNAc2 (minor). [UniProt]
Title Download Link
ARG81638 Mouse EGF ELISA Kit User manual download icon Download