ARG81911

Mouse FGF21 ELISA Kit

Mouse FGF21 ELISA Kit for ELISA and Mouse

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Component

Cat No Component Name Package Temp
ARG81911-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG81911-002 Standard 2 X 10 ng/vial 4°C
ARG81911-003 Standard/Sample diluent 30 ml (Ready to use) 4°C
ARG81911-004 Antibody conjugate concentrate (100X) 1 vial (100 µl) 4°C
ARG81911-005 Antibody diluent buffer 12 ml (Ready to use) 4°C
ARG81911-006 HRP-Streptavidin concentrate (100X) 1 vial (100 µl) 4°C
ARG81911-007 HRP-Streptavidin diluent buffer 12 ml (Ready to use) 4°C
ARG81911-008 25X Wash buffer 20 ml 4°C
ARG81911-009 TMB substrate 10 ml (Ready to use) 4°C (Protect from light)
ARG81911-010 STOP solution 10 ml (Ready to use) 4°C
ARG81911-011 Plate sealer 4 strips Room temperature

Overview

Product Description ARG81911 Mouse FGF21 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Mouse FGF21 in serum, plasma (heparin, EDTA) and cell culture supernatants.
Tested Reactivity Ms
Tested Application ELISA
Specificity There is no detectable cross-reactivity with other relevant proteins.
Target Name FGF21
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm.
Sensitivity 15.6 pg/ml
Sample Type Serum, plasma (heparin, EDTA) and cell culture supernatants.
Standard Range 31.2 - 2000 pg/ml
Sample Volume 100 µl
Precision Intra-Assay CV: 5.9%; Inter-Assay CV: 7.2%
Alternate Names Fibroblast growth factor 21; FGF-21

Application Instructions

Assay Time ~ 5 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 56636 Mouse FGF21

Swiss-port # Q9JJN1 Mouse Fibroblast growth factor 21

Gene Symbol FGF21
Gene Full Name fibroblast growth factor 21
Background The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities and are involved in a variety of biological processes including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. The function of this growth factor has not yet been determined. [provided by RefSeq, Jul 2008]
Function Stimulates glucose uptake in differentiated adipocytes via the induction of glucose transporter SLC2A1/GLUT1 expression (but not SLC2A4/GLUT4 expression). Activity requires the presence of KLB. [UniProt]
Highlight Related products:
FGF21 antibodies; FGF21 ELISA Kits; FGF21 recombinant proteins;
New ELISA data calculation tool:
Simplify the ELISA analysis by GainData

Specific References

PPARα agonist and metformin co-treatment ameliorates NASH in mice induced by a choline-deficient, amino acid-defined diet with 45% fat.

ELISA / Mouse / Serum

Shinya Okishio et al.
Sci Rep.,  (2020)

publication_link

 

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Title Download Link
ARG81911 Mouse FGF21 ELISA Kit User manual download icon Download