ARG70310
Mouse FLT3 Ligand recombinant protein (ECD) (Fc-His-tagged, C-ter)
Mouse FLT3 Ligand recombinant protein (ECD) (Fc-His-tagged, C-ter) for SDS-PAGE,Binding Activity and Mouse
Overview
| Product Description | HEK293 expressed, Fc-His-tagged (C-ter) Mouse FLT3 Ligand recombinant protein (ECD). |
|---|---|
| Tested Reactivity | Ms |
| Tested Application | Binding, SDS-PAGE |
| Target Name | FLT3 Ligand (ECD) |
| Species | Mouse |
| A.A. Sequence | Met1 - Arg188 of Mouse FLT3 Ligand (NP_038548.3.) with an Fc-6X His tag at the C-terminus. |
| Expression System | HEK293 |
| Alternate Names | FLT3L; SL cytokine; Fms-related tyrosine kinase 3 ligand; Flt3L; Flt3 ligand; FL |
Application Instructions
| Application Note | Binding activity test: Measured by its binding ability in a functional ELISA. Immobilized Recombinant Mouse FLT3, His Tag at 3µg/ml (100 µl/well) can bind Recombinant Mouse Flt-3 Ligand, The EC50 of Mouse Flt-3 Ligand is 36 ng/ml. |
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Properties
| Form | Powder |
|---|---|
| Purification Note | 0.22 µm filter sterilized. Endotoxin level is less than 0.1 EU/µg of the protein, as determined by the LAL test. |
| Purity | > 85% (by SDS-PAGE) |
| Buffer | PBS (pH 7.4) |
| Reconstitution | Reconstitute to a concentration of 0.1 - 0.5 mg/ml in sterile distilled water. |
| Storage Instruction | Lyophilized protein should be stored at -20°C or -80°C. After reconstitution, aliquot and store at -20°C for up to one month, at 2-8°C for up to one week. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Gene Symbol | FLT3LG |
|---|---|
| Gene Full Name | fms-related tyrosine kinase 3 ligand |
| Background | Dendritic cells (DCs) provide the key link between innate and adaptive immunity by recognizing pathogens and priming pathogen-specific immune responses. FLT3LG controls the development of DCs and is particularly important for plasmacytoid DCs and CD8 (see MIM 186910)-positive classical DCs and their CD103 (ITGAE; MIM 604682)-positive tissue counterparts (summary by Sathaliyawala et al., 2010 [PubMed 20933441]).[supplied by OMIM, Jan 2011] |
| Function | Stimulates the proliferation of early hematopoietic cells by activating FLT3. Synergizes well with a number of other colony stimulating factors and interleukins. [UniProt] |
| Cellular Localization | Isoform 1: Cell membrane; Single-pass type I membrane protein. Isoform 2: Secreted. [UniProt] |
| Calculated MW | 26 kDa |
