ARG70310

Mouse FLT3 Ligand recombinant protein (ECD) (Fc-His-tagged, C-ter)

Mouse FLT3 Ligand recombinant protein (ECD) (Fc-His-tagged, C-ter) for SDS-PAGE,Binding Activity and Mouse

Overview

Product Description HEK293 expressed, Fc-His-tagged (C-ter) Mouse FLT3 Ligand recombinant protein (ECD).
Tested Reactivity Ms
Tested Application Binding, SDS-PAGE
Target Name FLT3 Ligand (ECD)
Species Mouse
A.A. Sequence Met1 - Arg188 of Mouse FLT3 Ligand (NP_038548.3.) with an Fc-6X His tag at the C-terminus.
Expression System HEK293
Alternate Names FLT3L; SL cytokine; Fms-related tyrosine kinase 3 ligand; Flt3L; Flt3 ligand; FL

Application Instructions

Application Note Binding activity test: Measured by its binding ability in a functional ELISA. Immobilized Recombinant Mouse FLT3, His Tag at 3µg/ml (100 µl/well) can bind Recombinant Mouse Flt-3 Ligand, The EC50 of Mouse Flt-3 Ligand is 36 ng/ml.

Properties

Form Powder
Purification Note 0.22 µm filter sterilized. Endotoxin level is less than 0.1 EU/µg of the protein, as determined by the LAL test.
Purity > 85% (by SDS-PAGE)
Buffer PBS (pH 7.4)
Reconstitution Reconstitute to a concentration of 0.1 - 0.5 mg/ml in sterile distilled water.
Storage Instruction Lyophilized protein should be stored at -20°C or -80°C. After reconstitution, aliquot and store at -20°C for up to one month, at 2-8°C for up to one week. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Gene Symbol FLT3LG
Gene Full Name fms-related tyrosine kinase 3 ligand
Background Dendritic cells (DCs) provide the key link between innate and adaptive immunity by recognizing pathogens and priming pathogen-specific immune responses. FLT3LG controls the development of DCs and is particularly important for plasmacytoid DCs and CD8 (see MIM 186910)-positive classical DCs and their CD103 (ITGAE; MIM 604682)-positive tissue counterparts (summary by Sathaliyawala et al., 2010 [PubMed 20933441]).[supplied by OMIM, Jan 2011]
Function Stimulates the proliferation of early hematopoietic cells by activating FLT3. Synergizes well with a number of other colony stimulating factors and interleukins. [UniProt]
Cellular Localization Isoform 1: Cell membrane; Single-pass type I membrane protein. Isoform 2: Secreted. [UniProt]
Calculated MW 26 kDa