ARG81780

Mouse GDNF ELISA Kit

Mouse GDNF ELISA Kit for ELISA and Mouse

Component

Cat No Component Name Package Temp
ARG81780-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG81780-002 Standard 2 X 10 ng/vial 4°C
ARG81780-003 Standard/Sample diluent 30 ml (Ready to use) 4°C
ARG81780-004 Antibody conjugate concentrate (100X) 1 vial (100 µl) 4°C
ARG81780-005 Antibody diluent buffer 12 ml (Ready to use) 4°C
ARG81780-006 HRP-Streptavidin concentrate (100X) 1 vial (100 µl) 4°C
ARG81780-007 HRP-Streptavidin diluent buffer 12 ml (Ready to use) 4°C
ARG81780-008 25X Wash buffer 20 ml 4°C
ARG81780-009 TMB substrate 10 ml (Ready to use) 4°C (Protect from light)
ARG81780-010 STOP solution 10 ml (Ready to use) 4°C
ARG81780-011 Plate sealer 4 strips Room temperature

Overview

Product Description ARG81780 Mouse GDNF ELISA Kit is an Enzyme Immunoassay kit for the quantification of Mouse GDNF in serum, plasma (heparin, EDTA), cell culture supernatants and cell lysates.
Tested Reactivity Ms
Tested Application ELISA
Specificity There is no detectable cross-reactivity with other relevant proteins.
Target Name GDNF
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm.
Sensitivity 15.6 pg/ml
Sample Type Serum, plasma (heparin, EDTA), cell culture supernatants and cell lysates.
Standard Range 31.2 - 2000 pg/ml
Sample Volume 100 µl
Precision Intra-Assay CV: 6.2%; Inter-Assay CV: 6.5%
Alternate Names ATF; Astrocyte-derived trophic factor; HSCR3; hGDNF; Glial cell line-derived neurotrophic factor; HFB1-GDNF; ATF1; ATF2

Application Instructions

Assay Time ~ 5 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 14573 Mouse GDNF

Swiss-port # P48540 Mouse Glial cell line-derived neurotrophic factor

Gene Symbol GDNF
Gene Full Name glial cell derived neurotrophic factor
Background This gene encodes a highly conserved neurotrophic factor. The recombinant form of this protein was shown to promote the survival and differentiation of dopaminergic neurons in culture, and was able to prevent apoptosis of motor neurons induced by axotomy. The encoded protein is processed to a mature secreted form that exists as a homodimer. The mature form of the protein is a ligand for the product of the RET (rearranged during transfection) protooncogene. Multiple transcript variants encoding different isoforms have been found for this gene. Mutations in this gene may be associated with Hirschsprung disease. [provided by RefSeq, Jun 2010]
Function Neurotrophic factor that enhances survival and morphological differentiation of dopaminergic neurons and increases their high-affinity dopamine uptake. [UniProt]
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Title Download Link
ARG81780 Mouse GDNF ELISA Kit User's manual download icon Download