ARG82554

Mouse GDNF Receptor alpha 2 ELISA Kit

Mouse GDNF Receptor alpha 2 ELISA Kit for ELISA and Mouse

Component

Cat No Component Name Package Temp
ARG82554-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG82554-002 Standard 2 X 10 ng/vial 4°C
ARG82554-003 Standard/Sample diluent 30 ml (Ready to use) 4°C
ARG82554-004 Antibody conjugate concentrate (100X) 1 vial (100 µl) 4°C
ARG82554-005 Antibody diluent buffer 12 ml (Ready to use) 4°C
ARG82554-006 HRP-Streptavidin concentrate (100X) 1 vial (100 µl) 4°C
ARG82554-007 HRP-Streptavidin diluent buffer 12 ml (Ready to use) 4°C
ARG82554-008 25X Wash buffer 20 ml 4°C
ARG82554-009 TMB substrate 10 ml (Ready to use) 4°C (Protect from light)
ARG82554-010 STOP solution 10 ml (Ready to use) 4°C
ARG82554-011 Plate sealer 4 strips Room temperature

Overview

Product Description ARG82554 Mouse GDNF Receptor alpha 2 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Mouse GDNF Receptor alpha 2 in serum, plasma (EDTA, heparin, citrate) and cell culture supernatants.
Tested Reactivity Ms
Tested Application ELISA
Target Name GDNF Receptor alpha 2
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm.
Sensitivity 156 pg/ml
Sample Type Serum, plasma (EDTA, heparin, citrate) and cell culture supernatants.
Standard Range 312 - 20000 pg/ml
Sample Volume 100 µl
Precision Intra-Assay CV: 4.5%
Inter-Assay CV: 5.9%
Alternate Names NRTNR-alpha; GDNFR-alpha-2; NRTNR-ALPHA; GFR-alpha-2; GDNFRB; RET ligand 2; GDNF receptor alpha-2; GDNFR-beta; TRNR2; NTNRA; NTNR-alpha; TGF-beta-related neurotrophic factor receptor 2; RETL2; GDNF receptor beta; Neurturin receptor alpha; GDNF family receptor alpha-2

Application Instructions

Assay Time ~ 5 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 14586 Mouse GFRA2

Swiss-port # O08842 Mouse GDNF family receptor alpha-2

Gene Symbol GFRA2
Gene Full Name GDNF family receptor alpha 2
Background Glial cell line-derived neurotrophic factor (GDNF) and neurturin (NTN) are two structurally related, potent neurotrophic factors that play key roles in the control of neuron survival and differentiation. The protein encoded by this gene is a member of the GDNF receptor family. It is a glycosylphosphatidylinositol(GPI)-linked cell surface receptor for both GDNF and NTN, and mediates activation of the RET tyrosine kinase receptor. This encoded protein acts preferentially as a receptor for NTN compared to its other family member, GDNF family receptor alpha 1. This gene is a candidate gene for RET-associated diseases. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2009]
Function Receptor for neurturin. Mediates the NRTN-induced autophosphorylation and activation of the RET receptor. Also able to mediate GDNF signaling through the RET tyrosine kinase receptor.

Isoform 2: Participates to NRTN-induced 'Ser-727' phosphorylation of STAT3. [UniProt]
Cellular Localization Cell membrane; Lipid-anchor, GPI-anchor. [UniProt]
Highlight Related products:
GDNF Receptor antibodies; GDNF Receptor ELISA Kits;
New ELISA data calculation tool:
Simplify the ELISA analysis by GainData
Title Download Link
ARG82554 Mouse GDNF Receptor alpha 2 ELISA Kit User manual download icon Download