ARG70192
Mouse GM-CSF recombinant protein (Active) (His-tagged, N-ter)
Mouse GM-CSF recombinant protein (Active) (His-tagged, N-ter) for SDS-PAGE
Overview
| Product Description | E. coli expressed, His-tagged (N-ter) Active Mouse GM-CSF recombinant protein |
|---|---|
| Tested Application | SDS-PAGE |
| Target Name | GM-CSF |
| Species | Mouse |
| A.A. Sequence | APTRS PITVT RPWKH VEAIK EALNL LDDMP VTLNE EVEVV SNEFS FKKLT CVQTR LKIFE QGLRG NFTKL KGALN MTASY YQTYC PPTPE TDCET QVTTY ADFID SLKTF LTDIP FECKK PVQK with polyhistidine tag at the N-terminus. |
| Expression System | E. coli |
| Activity | Active |
| Activity Note | Determined by its ability to induce proliferation in FDC-P1 cells. The ED50 for this effect is < 50 pg/ml. The specific activity of recombinant mouse GM-CSF is approximately > 2x 10^7 IU/mg. |
| Alternate Names | GM-CSF; Granulocyte-macrophage colony-stimulating factor; CSF; GMCSF; Molgramostin; Colony-stimulating factor; Sargramostim |
Properties
| Form | Powder |
|---|---|
| Purification | Ni-NTA chromatography. |
| Purification Note | Endotoxin level is less than 0.1 EU/µg of the protein, as determined by the LAL test. |
| Purity | > 98% (by SDS-PAGE) |
| Buffer | PBS (pH 7.4) |
| Reconstitution | It is recommended to reconstitute the lyophilized protein in sterile water to a concentration not less than 100 µg/ml and incubate the stock solution for at least 20 min at room temperature to make sure the protein is dissolved completely. |
| Storage Instruction | Lyophilized protein should be stored at -20°C. After reconstitution, aliquot and store at -20°C or -80°C for up to one month. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Gene Symbol | CSF2 |
|---|---|
| Gene Full Name | colony stimulating factor 2 (granulocyte-macrophage) |
| Background | The protein encoded by this gene is a cytokine that controls the production, differentiation, and function of granulocytes and macrophages. The active form of the protein is found extracellularly as a homodimer. This gene has been localized to a cluster of related genes at chromosome region 5q31, which is known to be associated with interstitial deletions in the 5q- syndrome and acute myelogenous leukemia. Other genes in the cluster include those encoding interleukins 4, 5, and 13. [provided by RefSeq, Jul 2008] |
| Function | Cytokine that stimulates the growth and differentiation of hematopoietic precursor cells from various lineages, including granulocytes, macrophages, eosinophils and erythrocytes. [UniProt] |
| Cellular Localization | Secreted. [UniProt] |
| Highlight | Related products: GM-CSF antibodies; GM-CSF ELISA Kits; GM-CSF Duos / Panels; GM-CSF recombinant proteins; Related news: HMGB1 in inflammation Inflammatory Cytokines |
