ARG81891

Mouse GPNMB ELISA Kit

Mouse GPNMB ELISA Kit for ELISA and Mouse

Component

Cat No Component Name Package Temp
ARG81891-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG81891-002 Standard 2 X 10 ng/vial 4°C
ARG81891-003 Standard/Sample diluent 30 ml (Ready to use) 4°C
ARG81891-004 Antibody conjugate concentrate (100X) 1 vial (100 µl) 4°C
ARG81891-005 Antibody diluent buffer 12 ml (Ready to use) 4°C
ARG81891-006 HRP-Streptavidin concentrate (100X) 1 vial (100 µl) 4°C
ARG81891-007 HRP-Streptavidin diluent buffer 12 ml (Ready to use) 4°C
ARG81891-008 25X Wash buffer 20 ml 4°C
ARG81891-009 TMB substrate 10 ml (Ready to use) 4°C (Protect from light)
ARG81891-010 STOP solution 10 ml (Ready to use) 4°C
ARG81891-011 Plate sealer 4 strips Room temperature

Overview

Product Description ARG81891 Mouse GPNMB ELISA Kit is an Enzyme Immunoassay kit for the quantification of Mouse GPNMB in serum, plasma (heparin, EDTA) and cell culture supernatants.
Tested Reactivity Ms
Tested Application ELISA
Specificity There is no detectable cross-reactivity with other relevant proteins.
Target Name GPNMB
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm.
Sensitivity 78 pg/ml
Sample Type Serum, plasma (heparin, EDTA) and cell culture supernatants.
Standard Range 156 - 10000 pg/ml
Sample Volume 100 µl
Precision Intra-Assay CV: 5.3%; Inter-Assay CV: 6.7%
Alternate Names Transmembrane glycoprotein HGFIN; HGFIN; NMB; Transmembrane glycoprotein NMB

Application Instructions

Assay Time ~ 5 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 93695 Mouse GPNMB

Swiss-port # Q99P91 Mouse Transmembrane glycoprotein NMB

Gene Symbol GPNMB
Gene Full Name glycoprotein (transmembrane) nmb
Background The protein encoded by this gene is a type I transmembrane glycoprotein which shows homology to the pMEL17 precursor, a melanocyte-specific protein. GPNMB shows expression in the lowly metastatic human melanoma cell lines and xenografts but does not show expression in the highly metastatic cell lines. GPNMB may be involved in growth delay and reduction of metastatic potential. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Function Could be a melanogenic enzyme. [UniProt]
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Title Download Link
ARG81891 Mouse GPNMB ELISA Kit User manual download icon Download