ARG81736

Mouse Galectin 3 ELISA Kit

Mouse Galectin 3 ELISA Kit for ELISA and Mouse

Component

Cat No Component Name Package Temp
ARG81736-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG81736-002 Standard 2 X 10 ng/vial 4°C
ARG81736-003 Standard/Sample diluent 30 ml (Ready to use) 4°C
ARG81736-004 Antibody conjugate concentrate (100X) 1 vial (100 µl) 4°C
ARG81736-005 Antibody diluent buffer 12 ml (Ready to use) 4°C
ARG81736-006 HRP-Streptavidin concentrate (100X) 1 vial (100 µl) 4°C
ARG81736-007 HRP-Streptavidin diluent buffer 12 ml (Ready to use) 4°C
ARG81736-008 25X Wash buffer 20 ml 4°C
ARG81736-009 TMB substrate 10 ml (Ready to use) 4°C (Protect from light)
ARG81736-010 STOP solution 10 ml (Ready to use) 4°C
ARG81736-011 Plate sealer 4 strips Room temperature

Overview

Product Description ARG81736 Mouse Galectin 3 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Mouse Galectin 3 in serum, plasma (heparin, EDTA) and cell culture supernatants.
Tested Reactivity Ms
Tested Application ELISA
Specificity There is no detectable cross-reactivity with other relevant proteins.
Target Name Galectin 3
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm.
Sensitivity 31.25 pg/ml
Sample Type Serum, plasma (heparin, EDTA) and cell culture supernatants.
Standard Range 62.5 - 4000 pg/ml
Sample Volume 100 µl
Precision Intra-Assay CV: 7.6%; Inter-Assay CV: 8.9%
Alternate Names Laminin-binding protein; Gal-3; L-31; GALBP; Galactoside-binding protein; MAC2; GAL3; GALIG; Mac-2 antigen; CBP 35; Galectin-3; CBP35; Galactose-specific lectin 3; IgE-binding protein; L31; 35 kDa lectin; Carbohydrate-binding protein 35; Lectin L-29

Application Instructions

Assay Time ~ 5 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Gene Symbol LGALS3
Gene Full Name lectin, galactoside-binding, soluble, 3
Background This gene encodes a member of the galectin family of carbohydrate binding proteins. Members of this protein family have an affinity for beta-galactosides. The encoded protein is characterized by an N-terminal proline-rich tandem repeat domain and a single C-terminal carbohydrate recognition domain. This protein can self-associate through the N-terminal domain allowing it to bind to multivalent saccharide ligands. This protein localizes to the extracellular matrix, the cytoplasm and the nucleus. This protein plays a role in numerous cellular functions including apoptosis, innate immunity, cell adhesion and T-cell regulation. The protein exhibits antimicrobial activity against bacteria and fungi. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Oct 2014]
Function Galactose-specific lectin which binds IgE. May mediate with the alpha-3, beta-1 integrin the stimulation by CSPG4 of endothelial cells migration. Together with DMBT1, required for terminal differentiation of columnar epithelial cells during early embryogenesis (By similarity). In the nucleus: acts as a pre-mRNA splicing factor. Involved in acute inflammatory responses including neutrophil activation and adhesion, chemoattraction of monocytes macrophages, opsonization of apoptotic neutrophils, and activation of mast cells. [UniProt]
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Title Download Link
ARG81736 Mouse Galectin 3 ELISA Kit User's manual download icon Download