ARG81811

Mouse Granzyme B ELISA Kit

Mouse Granzyme B ELISA Kit for ELISA and Mouse

Component

Cat No Component Name Package Temp
ARG81811-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG81811-002 Standard 2 X 10 ng/vial 4°C
ARG81811-003 Standard/Sample diluent 30 ml (Ready to use) 4°C
ARG81811-004 Antibody conjugate concentrate (100X) 1 vial (100 µl) 4°C
ARG81811-005 Antibody diluent buffer 12 ml (Ready to use) 4°C
ARG81811-006 HRP-Streptavidin concentrate (100X) 1 vial (100 µl) 4°C
ARG81811-007 HRP-Streptavidin diluent buffer 12 ml (Ready to use) 4°C
ARG81811-008 25X Wash buffer 20 ml 4°C
ARG81811-009 TMB substrate 10 ml (Ready to use) 4°C (Protect from light)
ARG81811-010 STOP solution 10 ml (Ready to use) 4°C
ARG81811-011 Plate sealer 4 strips Room temperature

Overview

Product Description ARG81811 Mouse Granzyme B ELISA Kit is an Enzyme Immunoassay kit for the quantification of Mouse Granzyme B in serum, plasma (heparin, EDTA) and cell culture supernatants.
Tested Reactivity Ms
Tested Application ELISA
Specificity There is no detectable cross-reactivity with other relevant proteins.
Target Name Granzyme B
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm.
Sensitivity 7.8 pg/ml
Sample Type Serum, plasma (heparin, EDTA) and cell culture supernatants.
Standard Range 15.6 - 1000 pg/ml
Sample Volume 100 µl
Precision Intra-Assay CV: 5.9%; Inter-Assay CV: 7.2%
Alternate Names EC 3.4.21.79; CTLA-1; CSP-B; Granzyme B; CTLA1; CCPI; CGL-1; CGL1; Cytotoxic T-lymphocyte proteinase 2; T-cell serine protease 1-3E; HLP; CTSGL1; in-2; Cathepsin G-like 1; SECT; Granzyme-2; Human lymphocyte protein; C11; CSPB; Lymphocyte protease

Application Instructions

Assay Time ~ 5 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 14939 Mouse GZMB

Swiss-port # P04187 Mouse Granzyme B(G,H)

Gene Symbol GZMB
Gene Full Name granzyme B (granzyme 2, cytotoxic T-lymphocyte-associated serine esterase 1)
Background Granzyme B is a member of the granzyme subfamily of proteins, part of the peptidase S1 family of serine proteases. The encoded preproprotein is secreted by natural killer (NK) cells and cytotoxic T lymphocytes (CTLs) and proteolytically processed to generate the active protease, which induces target cell apoptosis. This protein also processes cytokines and degrades extracellular matrix proteins, and these roles are implicated in chronic inflammation and wound healing. Expression of this gene may be elevated in human patients with cardiac fibrosis. [provided by RefSeq, Sep 2016]
Function Granzyme B is necessary for target cell lysis in cell-mediated immune responses. It cleaves after Asp. Seems to be linked to an activation cascade of caspases (aspartate-specific cysteine proteases) responsible for apoptosis execution. Cleaves caspase-3, -7, -9 and 10 to give rise to active enzymes mediating apoptosis. [UniProt]
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Title Download Link
ARG81811 Mouse Granzyme B ELISA Kit User's manual download icon Download