ARG81738

Mouse HGFA Inhibitor 1 ELISA Kit

Mouse HGFA Inhibitor 1 ELISA Kit for ELISA and Mouse

Component

Cat No Component Name Package Temp
ARG81738-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG81738-002 Standard 2 X 10 ng/vial 4°C
ARG81738-003 Standard/Sample diluent 30 ml (Ready to use) 4°C
ARG81738-004 Antibody conjugate concentrate (100X) 1 vial (100 µl) 4°C
ARG81738-005 Antibody diluent buffer 12 ml (Ready to use) 4°C
ARG81738-006 HRP-Streptavidin concentrate (100X) 1 vial (100 µl) 4°C
ARG81738-007 HRP-Streptavidin diluent buffer 12 ml (Ready to use) 4°C
ARG81738-008 25X Wash buffer 20 ml 4°C
ARG81738-009 TMB substrate 10 ml (Ready to use) 4°C (Protect from light)
ARG81738-010 STOP solution 10 ml (Ready to use) 4°C
ARG81738-011 Plate sealer 4 strips Room temperature

Overview

Product Description ARG81738 Mouse HGFA Inhibitor 1 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Mouse HGFA Inhibitor 1 in serum, plasma (heparin) and cell culture supernatants.
Tested Reactivity Ms
Tested Application ELISA
Specificity There is no detectable cross-reactivity with other relevant proteins.
Target Name HGFA Inhibitor 1
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm.
Sensitivity 31.25 pg/ml
Sample Type Serum, plasma (heparin) and cell culture supernatants.
Standard Range 62.5 - 4000 pg/ml
Sample Volume 100 µl
Precision Intra-Assay CV: 6.5%; Inter-Assay CV: 7.6%
Alternate Names HAI; Kunitz-type protease inhibitor 1; HAI1; HAI-1; MANSC2; Hepatocyte growth factor activator inhibitor type 1

Application Instructions

Assay Time ~ 5 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 20732 Mouse SPINT1

Swiss-port # Q9R097 Mouse Kunitz-type protease inhibitor 1

Gene Symbol SPINT1
Gene Full Name serine peptidase inhibitor, Kunitz type 1
Background The protein encoded by this gene is a member of the Kunitz family of serine protease inhibitors. The protein is a potent inhibitor specific for HGF activator and is thought to be involved in the regulation of the proteolytic activation of HGF in injured tissues. Alternative splicing results in multiple variants encoding different isoforms. [provided by RefSeq, Jul 2008]
Function Inhibitor of HGF activator. Also acts as an inhibitor of matriptase (ST14). [UniProt]
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Title Download Link
ARG81738 Mouse HGFA Inhibitor 1 ELISA Kit User's manual download icon Download