ARG70226
Mouse IFN beta 1a recombinant protein (Active) (His-tagged, C-ter)
Mouse IFN beta 1a recombinant protein (Active) (His-tagged, C-ter) for SDS-PAGE and Mouse
Overview
| Product Description | E. coli expressed, His-tagged (C-ter) Active Mouse IFN beta 1a recombinant protein. |
|---|---|
| Tested Reactivity | Ms |
| Tested Application | SDS-PAGE |
| Target Name | IFN beta 1a |
| Species | Mouse |
| A.A. Sequence | MINYK QLQLQ ERTNI RKCQE LLEQL NGKIN LTYRA DFKIP MEMTE KMQKS YTAFA IQEML QNVFL VFRNF SSTGW NETIV VRLLD ELHQQ TVFLK TVLEE KQEER LTWEM SSTAL HLKSY YWRVQ RYLKL MKYNS YAWMV VRAEI FRNFL IIRRL TRNFQ N with polyhistidine tag at the C-terminus. |
| Expression System | E. coli |
| Activity | Active |
| Activity Note | Measure by its ability to protect HeLa cells infected with encephalomyocarditis (EMC) virus. The ED50 for this effect is < 5 pg/ml. The specific activity of recombinant mouse IFN beta 1a is > 1 x 10^9 IU/mg. |
| Alternate Names | Fibroblast interferon; IFN-beta; IFNB; IFF; IFB; Interferon beta |
Application Instructions
| Observed Size | ~ 20 kDa |
|---|
Properties
| Form | Powder |
|---|---|
| Purification | Ni-NTA chromatography. |
| Purification Note | Endotoxin level is less than 0.1 EU/µg of the protein, as determined by the LAL test. |
| Purity | > 98% (by SDS-PAGE) |
| Buffer | PBS (pH 7.4) |
| Reconstitution | It is recommended to reconstitute the lyophilized protein in sterile water to a concentration not less than 100 µg/ml and incubate the stock solution for at least 20 min at room temperature to make sure the protein is dissolved completely. |
| Storage Instruction | Lyophilized protein should be stored at -20°C. After reconstitution, aliquot and store at -20°C or -80°C for up to one month. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | IFNB1 |
| Gene Full Name | interferon, beta 1, fibroblast |
| Background | This gene encodes a cytokine that belongs to the interferon family of signaling proteins, which are released as part of the innate immune response to pathogens. The protein encoded by this gene belongs to the type I class of interferons, which are important for defense against viral infections. In addition, type I interferons are involved in cell differentiation and anti-tumor defenses. Following secretion in response to a pathogen, type I interferons bind a homologous receptor complex and induce transcription of genes such as those encoding inflammatory cytokines and chemokines. Overactivation of type I interferon secretion is linked to autoimmune diseases. Mice deficient for this gene display several phenotypes including defects in B cell maturation and increased susceptibility to viral infection. [provided by RefSeq, Sep 2015] |
| Function | Has antiviral, antibacterial and anticancer activities. [UniProt] |
| Cellular Localization | Secreted. [UniProt] |
| Highlight | Related products: IFN beta antibodies; IFN beta ELISA Kits; IFN beta recombinant proteins; Related news: circNDUFB2, a circular RNA (circRNA), activates anti-tumor immunity |
| Calculated MW | 22 kDa |
