ARG80204

Mouse IL1 alpha ELISA Kit

Mouse IL1 alpha ELISA Kit for ELISA and Mouse

Immune System kit; Metabolism kit
publication_link Publication1

Component

Cat No Component Name Package Temp
ARG80204-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG80204-002 Standard (Lyophilized) 3 X 1 ng/vial 4°C
ARG80204-003 Standard diluent buffer 20 ml 4°C
ARG80204-004 Antibody conjugate concentrate 400 µl 4°C
ARG80204-005 Antibody diluent buffer 16 ml 4°C
ARG80204-006 HRP-Streptavidin concentrate 400 µl 4°C (Protect from light)
ARG80204-007 HRP-Streptavidin diluent buffer 16 ml 4°C
ARG80204-008 20X Wash buffer 50 ml 4°C
ARG80204-009 TMB substrate 12ml 4°C (Protect from light)
ARG80204-010 STOP solution 12ml 4°C
ARG80204-011 Plate sealer 4 strips Room temperature

Overview

Product Description

ARG80204 Mouse IL1 alpha ELISA Kit is an Enzyme Immunoassay kit for the quantification of Mouse IL1 alpha in Serum, Plasma, Cell culture supernatants.

Tested Reactivity Ms
Tested Application ELISA
Specificity No significant cross-reactivity or interference with Human IL-1 alpha
Target Name IL1 alpha
Conjugation HRP
Conjugation Note

Substrate: TMB and read at 450 nm

Sensitivity 15 pg/ml
Sample Type Serum, Plasma, Cell culture supernatants
Standard Range 31.25 - 2000 pg/ml
Sample Volume 100 µl
Precision

CV: <10%

Alternate Names IL-1 alpha; Interleukin-1 alpha; IL1; IL1F1; Hematopoietin-1; IL1-ALPHA; IL-1A

Application Instructions

Assay Time 4 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 16175 Mouse IL1A

Swiss-port # P01582 Mouse Interleukin-1 alpha

Gene Symbol Il1a
Gene Full Name interleukin 1 alpha
Background The protein encoded by this gene is a member of the interleukin 1 cytokine family. This cytokine is a pleiotropic cytokine involved in various immune responses, inflammatory processes, and hematopoiesis. This cytokine is produced by monocytes and macrophages as a proprotein, which is proteolytically processed and released in response to cell injury, and thus induces apoptosis. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. It has been suggested that the polymorphism of these genes is associated with rheumatoid arthritis and Alzheimer's disease. [provided by RefSeq]
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Research Area Immune System kit; Metabolism kit

Specific References

Porphyromonas gingivalis induces depression via downregulating p75NTR-mediated BDNF maturation in astrocytes.

ELISA / Mouse Brain tissue lysate

Wang Yi-Xi et al.
Brain Behav Immun.,  (2019)

publication_link

 

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Title Download Link
ARG80204 Mouse IL1 alpha ELISA Kit User's manual download icon Download