ARG80200
Mouse IL10 ELISA Kit
Mouse IL10 ELISA Kit for ELISA and Mouse
Cancer kit; Immune System kit
Publication5
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG80200-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG80200-002 | Standard (Lyophilized) | 3 X 0.5 ng/vial | 4°C |
| ARG80200-003 | Standard diluent buffer | 20 ml | 4°C |
| ARG80200-004 | Antibody conjugate concentrate | 400 µl | 4°C |
| ARG80200-005 | Antibody diluent buffer | 16 ml | 4°C |
| ARG80200-006 | HRP-Streptavidin concentrate | 400 µl | 4°C (Protect from light) |
| ARG80200-007 | HRP-Streptavidin diluent buffer | 16 ml | 4°C |
| ARG80200-008 | 20X Wash buffer | 50 ml | 4°C |
| ARG80200-009 | TMB substrate | 12ml | 4°C (Protect from light) |
| ARG80200-010 | STOP solution | 12ml | 4°C |
| ARG80200-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG80200 Mouse IL10 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Mouse IL10 in Serum, Plasma, Cell culture supernatants. |
|---|---|
| Tested Reactivity | Ms |
| Tested Application | ELISA |
| Specificity | No significant cross-reactivity or interference with Human IL-10, IL-10R；mouse IL-10R；canine IL-10；feline IL-10；porcine IL-10 |
| Target Name | IL10 |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm |
| Sensitivity | 2 pg/ml |
| Sample Type | Serum, Plasma, Cell culture supernatants |
| Standard Range | 3.9 - 250 pg/ml |
| Sample Volume | 100 µl |
| Precision | CV: <10% |
| Alternate Names | IL10A; GVHDS; TGIF; IL-10; Cytokine synthesis inhibitory factor; CSIF; Interleukin-10 |
Application Instructions
| Assay Time | 4 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | Il10 |
| Gene Full Name | interleukin 10 |
| Background | The protein encoded by this gene is a cytokine produced primarily by monocytes and to a lesser extent by lymphocytes. This cytokine has pleiotropic effects in immunoregulation and inflammation. It down-regulates the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. This cytokine can block NF-kappa B activity, and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice suggested the function of this cytokine as an essential immunoregulator in the intestinal tract. [provided by RefSeq] |
| Highlight | Related products: IL10 antibodies; IL10 ELISA Kits; IL10 recombinant proteins; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| Research Area | Cancer kit; Immune System kit |
Specific References
ACTH4-10 protects the ADR-injuried podocytes by stimulating B lymphocytes to secrete IL-10.
ELISA / Mouse
Influenza vaccination in early Alzheimer's disease rescues amyloidosis and ameliorates cognitive deficits in APP/PS1 mice by inhibiting regulatory T cells.
ELISA / Mouse
Early label-free analysis of mitochondrial redox states by Raman spectroscopy predicts septic outcomes
ELISA / Mouse
Dietary polysaccharide-rich extract from Eucheuma cottonii modulates the inflammatory response and suppresses colonic injury on dextran sulfate sodium-induced colitis in mice.
ELISA / Mouse
Red Seaweed Eucheuma cottonii Ethanol Extract Regulates Inflammatory Mediators Production and Prevents Colonic Injury on Acute Colitis Disease in Mice
ELISA / Mouse
| Title | Download Link |
|---|---|
| ARG80200 Mouse IL10 ELISA Kit User's manual |
 Download
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