ARG81655

Mouse IL11 ELISA Kit

Mouse IL11 ELISA Kit for ELISA and Mouse

Component

Cat No Component Name Package Temp
ARG81655-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG81655-002 Standard 2 X 10 ng/vial 4°C
ARG81655-003 Standard/Sample diluent 30 ml (Ready to use) 4°C
ARG81655-004 Antibody conjugate concentrate (100X) 1 vial (100 µl) 4°C
ARG81655-005 Antibody diluent buffer 12 ml (Ready to use) 4°C
ARG81655-006 HRP-Streptavidin concentrate (100X) 1 vial (100 µl) 4°C
ARG81655-007 HRP-Streptavidin diluent buffer 12 ml (Ready to use) 4°C
ARG81655-008 25X Wash buffer 20 ml 4°C
ARG81655-009 TMB substrate 10 ml (Ready to use) 4°C (Protect from light)
ARG81655-010 STOP solution 10 ml (Ready to use) 4°C
ARG81655-011 Plate sealer 4 strips Room temperature

Overview

Product Description ARG81655 Mouse IL11 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Mouse IL11 in serum, plasma (heparin, EDTA, citrate) and cell culture supernatants.
Tested Reactivity Ms
Tested Application ELISA
Specificity There is no detectable cross-reactivity with other relevant proteins.
Target Name IL11
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm.
Sensitivity 15.6 pg/ml
Sample Type Serum, plasma (heparin, EDTA, citrate) and cell culture supernatants.
Standard Range 31.2 - 2000 pg/ml
Sample Volume 100 µl
Precision Intra-Assay CV: 6.7%; Inter-Assay CV: 7.2%
Alternate Names Oprelvekin; Interleukin-11; AGIF; Adipogenesis inhibitory factor; IL-11

Application Instructions

Assay Time ~ 5 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 16156 Mouse IL11

Swiss-port # P47873 Mouse Interleukin-11

Gene Symbol IL11
Gene Full Name interleukin 11
Background The protein encoded by this gene is a member of the gp130 family of cytokines. These cytokines drive the assembly of multisubunit receptor complexes, all of which contain at least one molecule of the transmembrane signaling receptor IL6ST (gp130). This cytokine is shown to stimulate the T-cell-dependent development of immunoglobulin-producing B cells. It is also found to support the proliferation of hematopoietic stem cells and megakaryocyte progenitor cells. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jun 2012]
Function Cytokine that stimulates the proliferation of hematopoietic stem cells and megakaryocyte progenitor cells and induces megakaryocyte maturation resulting in increased platelet production. Also promotes the proliferation of hepatocytes in response to liver damage. Binding to its receptor formed by IL6ST and either IL11RA1 or IL11RA2 activates a signaling cascade that promotes cell proliferation. Signaling leads to the activation of intracellular protein kinases and the phosphorylation of STAT3. [UniProt]
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Title Download Link
ARG81655 Mouse IL11 ELISA Kit User manual download icon Download