ARG70165
Mouse IL13 recombinant protein (Active) (His-tagged, C-ter)
Mouse IL13 recombinant protein (Active) (His-tagged, C-ter) for SDS-PAGE
Overview
| Product Description | E. coli expressed, His-tagged (C-ter) Active Mouse IL13 recombinant protein |
|---|---|
| Tested Application | SDS-PAGE |
| Target Name | IL13 |
| Species | Mouse |
| A.A. Sequence | MPVPR SVSLP LTLKE LIEEL SNITQ DQTPL CNGSM VWSVD LAAGG FCVAL DSLTN ISNCN AIYRT QRILH GLCNR KAPTT VSSLP DTKIE VAHFI TKLLS YTKQL FRHGP F with polyhistidine tag at the C-terminus. |
| Expression System | E. coli |
| Activity | Active |
| Activity Note | Determined by its ability to induce TF-1 cells proliferation. The ED50 for this effect is < 4 ng/ml. The specific activity of recombinant mouse IL-13 is > 2.5 x 10^5 IU/mg. |
| Alternate Names | Interleukin-13; P600; IL-13 |
Properties
| Form | Powder |
|---|---|
| Purification | Ni-NTA chromatography. |
| Purification Note | Endotoxin level is less than 0.1 EU/µg of the protein, as determined by the LAL test. |
| Purity | > 95% (by SDS-PAGE) |
| Buffer | PBS (pH 7.4) |
| Reconstitution | It is recommended to reconstitute the lyophilized protein in sterile water to a concentration not less than 100 µg/ml and incubate the stock solution for at least 20 min at room temperature to make sure the protein is dissolved completely. |
| Storage Instruction | Lyophilized protein should be stored at -20°C. After reconstitution, aliquot and store at -20°C or -80°C for up to one month. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Gene Symbol | IL13 |
|---|---|
| Gene Full Name | interleukin 13 |
| Background | This gene encodes an immunoregulatory cytokine produced primarily by activated Th2 cells. This cytokine is involved in several stages of B-cell maturation and differentiation. It up-regulates CD23 and MHC class II expression, and promotes IgE isotype switching of B cells. This cytokine down-regulates macrophage activity, thereby inhibits the production of pro-inflammatory cytokines and chemokines. This cytokine is found to be critical to the pathogenesis of allergen-induced asthma but operates through mechanisms independent of IgE and eosinophils. This gene, IL3, IL5, IL4, and CSF2 form a cytokine gene cluster on chromosome 5q, with this gene particularly close to IL4. [provided by RefSeq, Jul 2008] |
| Function | Cytokine. Inhibits inflammatory cytokine production. Synergizes with IL2 in regulating interferon-gamma synthesis. May be critical in regulating inflammatory and immune responses. [UniProt] |
| Cellular Localization | Secreted. [UniProt] |
