ARG81742

Mouse IL1RA ELISA Kit

Mouse IL1RA ELISA Kit for ELISA and Mouse

Component

Cat No Component Name Package Temp
ARG81742-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG81742-002 Standard 2 X 10 ng/vial 4°C
ARG81742-003 Standard/Sample diluent 30 ml (Ready to use) 4°C
ARG81742-004 Antibody conjugate concentrate (100X) 1 vial (100 µl) 4°C
ARG81742-005 Antibody diluent buffer 12 ml (Ready to use) 4°C
ARG81742-006 HRP-Streptavidin concentrate (100X) 1 vial (100 µl) 4°C
ARG81742-007 HRP-Streptavidin diluent buffer 12 ml (Ready to use) 4°C
ARG81742-008 25X Wash buffer 20 ml 4°C
ARG81742-009 TMB substrate 10 ml (Ready to use) 4°C (Protect from light)
ARG81742-010 STOP solution 10 ml (Ready to use) 4°C
ARG81742-011 Plate sealer 4 strips Room temperature

Overview

Product Description ARG81742 Mouse IL1RA ELISA Kit is an Enzyme Immunoassay kit for the quantification of Mouse IL1RA in serum, plasma (heparin, EDTA) and cell culture supernatants.
Tested Reactivity Ms
Tested Application ELISA
Specificity There is no detectable cross-reactivity with other relevant proteins.
Target Name IL1RA
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm.
Sensitivity 31.25 pg/ml
Sample Type Serum, plasma (heparin, EDTA) and cell culture supernatants.
Standard Range 62.5 - 4000 pg/ml
Sample Volume 100 µl
Precision Intra-Assay CV: 5.3%; Inter-Assay CV: 6.7%
Alternate Names IL1 inhibitor; Interleukin-1 receptor antagonist protein; ICIL-1RA; MVCD4; IL1RA; DIRA; IL-1RN; Anakinra; IL1F3; IRAP; IL-1ra; IL-1ra3

Application Instructions

Assay Time ~ 5 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 16181 Mouse IL1RN

Swiss-port # P25085 Mouse Interleukin-1 receptor antagonist protein

Gene Symbol IL1RN
Gene Full Name interleukin 1 receptor antagonist
Background The protein encoded by this gene is a member of the interleukin 1 cytokine family. This protein inhibits the activities of interleukin 1, alpha (IL1A) and interleukin 1, beta (IL1B), and modulates a variety of interleukin 1 related immune and inflammatory responses. This gene and five other closely related cytokine genes form a gene cluster spanning approximately 400 kb on chromosome 2. A polymorphism of this gene is reported to be associated with increased risk of osteoporotic fractures and gastric cancer. Four alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008]
Function Inhibits the activity of interleukin-1 by binding to receptor IL1R1 and preventing its association with the coreceptor IL1RAP for signaling. Has no interleukin-1 like activity. Binds functional interleukin-1 receptor IL1R1 with greater affinity than decoy receptor IL1R2; however, the physiological relevance of the latter association is unsure. [UniProt]
Highlight Related products:
IL1RA antibodies; IL1RA ELISA Kits; IL1RA recombinant proteins;
New ELISA data calculation tool:
Simplify the ELISA analysis by GainData
Title Download Link
ARG81742 Mouse IL1RA ELISA Kit User's manual download icon Download