ARG70154
Mouse IL2 recombinant protein (Active) (His-tagged, C-ter)
Mouse IL2 recombinant protein (Active) (His-tagged, C-ter) for SDS-PAGE
Overview
| Product Description | E. coli expressed, His-tagged (C-ter) Active Mouse IL2 recombinant protein |
|---|---|
| Tested Application | SDS-PAGE |
| Target Name | IL2 |
| Species | Mouse |
| A.A. Sequence | MAPTS SSTSS STAEA QQQQQ QQQQQ QQHLE QLLMD LQELL SRMEN YRNLK LPRML TFKFY LPKQA TELKD LQCLE DELGP LRHVL DLTQS KSFQL EDAEN FISNI RVTVV KLKGS DNTFE CQFDD ESATV VDFLR RWIAF CQSII STSPQ with polyhistidine tag at the C-terminus. |
| Expression System | E. coli |
| Activity | Active |
| Activity Note | Determined by its ability to induce CTLL-2 cells proliferation. The ED50 for this effect is < 0.3 ng/ml. The specific activity of recombinant mouse IL-2 is approximately > 3 x 10^6 IU/mg. |
| Alternate Names | TCGF; IL-2; lymphokine; Interleukin-2; Aldesleukin; T-cell growth factor |
Properties
| Form | Powder |
|---|---|
| Purification | Ni-NTA chromatography. |
| Purification Note | Endotoxin level is less than 0.1 EU/µg of the protein, as determined by the LAL test. |
| Purity | > 98% (by SDS-PAGE) |
| Buffer | PBS (pH 7.4) |
| Reconstitution | It is recommended to reconstitute the lyophilized protein in sterile water to a concentration not less than 100 µg/ml and incubate the stock solution for at least 20 min at room temperature to make sure the protein is dissolved completely. |
| Storage Instruction | Lyophilized protein should be stored at -20°C. After reconstitution, aliquot and store at -20°C or -80°C for up to one month. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Gene Symbol | IL2 |
|---|---|
| Gene Full Name | interleukin 2 |
| Background | The protein encoded by this gene is a secreted cytokine that is important for the proliferation of T and B lymphocytes. The receptor of this cytokine is a heterotrimeric protein complex whose gamma chain is also shared by interleukin 4 (IL4) and interleukin 7 (IL7). The expression of this gene in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. The targeted disruption of a similar gene in mice leads to ulcerative colitis-like disease, which suggests an essential role of this gene in the immune response to antigenic stimuli. [provided by RefSeq, Jul 2008] |
| Function | Produced by T-cells in response to antigenic or mitogenic stimulation, this protein is required for T-cell proliferation and other activities crucial to regulation of the immune response. Can stimulate B-cells, monocytes, lymphokine-activated killer cells, natural killer cells, and glioma cells. [UniProt] |
| Cellular Localization | Secreted. [UniProt] |
