ARG70315
Mouse IL21R recombinant protein (ECD) (His-tagged, C-ter)
Mouse IL21R recombinant protein (ECD) (His-tagged, C-ter) for SDS-PAGE,Binding Activity and Mouse
Overview
| Product Description | HEK293 expressed, His-tagged (C-ter) Mouse IL21R recombinant protein (ECD). |
|---|---|
| Tested Reactivity | Ms |
| Tested Application | Binding, SDS-PAGE |
| Target Name | IL21R (ECD) |
| Species | Mouse |
| A.A. Sequence | Cys20 - Pro236 of Mouse IL21R (NP_068687.1) with 6X His tag at the C-terminus. |
| Expression System | HEK293 |
| Alternate Names | Interleukin-21 receptor; Novel interleukin receptor; CD360; CD antigen CD360; NILR; IL-21R; IL-21 receptor |
Application Instructions
| Application Note | Binding activity test: Measured by its binding ability in a functional ELISA. Immobilized Recombinant Mouse IL21R at 2µg/ml (100µl/well) can bind Recombinant Human IL-21 with a linear range of 0.128-83.57 ng/ml. |
|---|
Properties
| Form | Powder |
|---|---|
| Purification Note | 0.22 µm filter sterilized. Endotoxin level is less than 0.1 EU/µg of the protein, as determined by the LAL test. |
| Purity | > 97% (by SDS-PAGE) |
| Buffer | PBS (pH 7.4) |
| Reconstitution | Reconstitute to a concentration of 0.1 - 0.5 mg/ml in sterile distilled water. |
| Storage Instruction | Lyophilized protein should be stored at -20°C or -80°C. After reconstitution, aliquot and store at -20°C for up to one month, at 2-8°C for up to one week. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | IL21R |
| Gene Full Name | interleukin 21 receptor |
| Background | The protein encoded by this gene is a cytokine receptor for interleukin 21 (IL21). It belongs to the type I cytokine receptors, and has been shown to form a heterodimeric receptor complex with the common gamma-chain, a receptor subunit also shared by the receptors for interleukin 2, 4, 7, 9, and 15. This receptor transduces the growth promoting signal of IL21, and is important for the proliferation and differentiation of T cells, B cells, and natural killer (NK) cells. The ligand binding of this receptor leads to the activation of multiple downstream signaling molecules, including JAK1, JAK3, STAT1, and STAT3. Knockout studies of a similar gene in mouse suggest a role for this gene in regulating immunoglobulin production. Three alternatively spliced transcript variants have been described. [provided by RefSeq, Jul 2010] |
| Function | This is a receptor for interleukin-21. [UniProt] |
| Cellular Localization | Membrane; Single-pass type I membrane protein. [UniProt] |
| Calculated MW | 59 kDa |
| PTM | C-mannosylated at Trp-214 in the WSXWS motif, the sugar chain makes extensive hydrogen bonds with Asn-73 sugar, and bridges the two fibronectin domains transforming the V-shaped receptor into an A-frame. [UniProt] |
