ARG70173

Mouse IL22 recombinant protein (Active) (His-tagged, C-ter)

Mouse IL22 recombinant protein (Active) (His-tagged, C-ter) for SDS-PAGE

Overview

Product Description E. coli expressed, His-tagged (C-ter) Active Mouse IL22 recombinant protein
Tested Application SDS-PAGE
Target Name IL22
Species Mouse
A.A. Sequence MLPVN TRCKL EVSNF QQPYI VNRTF MLAKE ASLAD NNTDV RLIGE KLFRG VSAK DQCYL MKQVL NFTLE DVLLP QSDRF QPYMQ EVVPF LTKLS NQLSS CHISG DDQ N IQKNV RRLKE TVKKL GESGE IKAIG ELDLL FMSLR NACV with polyhistidine tag at the C-terminus.
Expression System E. coli
Activity Active
Activity Note Determined by its ability to induce IL-10 secretion in COLO205 cells. The ED50 for this effect is < 0.3 ng/ml.
Alternate Names Interleukin-22; zcyto18; IL-TIF; IL-22; IL-21; IL-D110; TIFa; ILTIF; TIFIL-23; IL-10-related T-cell-derived-inducible factor; Cytokine Zcyto18

Properties

Form Powder
Purification Ni-NTA chromatography.
Purification Note Endotoxin level is less than 0.1 EU/µg of the protein, as determined by the LAL test.
Purity > 98% (by SDS-PAGE)
Buffer PBS (pH 7.4)
Reconstitution It is recommended to reconstitute the lyophilized protein in sterile water to a concentration not less than 100 µg/ml and incubate the stock solution for at least 20 min at room temperature to make sure the protein is dissolved completely.
Storage Instruction Lyophilized protein should be stored at -20°C. After reconstitution, aliquot and store at -20°C or -80°C for up to one month. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Gene Symbol IL22
Gene Full Name interleukin 22
Function Cytokine that contributes to the inflammatory response in vivo. [UniProt]
Cellular Localization Secreted. [UniProt]