ARG81900

Mouse IL24 ELISA Kit

Mouse IL24 ELISA Kit for ELISA and Mouse

Component

Cat No Component Name Package Temp
ARG81900-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG81900-002 Standard 2 X 10 ng/vial 4°C
ARG81900-003 Standard/Sample diluent 30 ml (Ready to use) 4°C
ARG81900-004 Antibody conjugate concentrate (100X) 1 vial (100 µl) 4°C
ARG81900-005 Antibody diluent buffer 12 ml (Ready to use) 4°C
ARG81900-006 HRP-Streptavidin concentrate (100X) 1 vial (100 µl) 4°C
ARG81900-007 HRP-Streptavidin diluent buffer 12 ml (Ready to use) 4°C
ARG81900-008 25X Wash buffer 20 ml 4°C
ARG81900-009 TMB substrate 10 ml (Ready to use) 4°C (Protect from light)
ARG81900-010 STOP solution 10 ml (Ready to use) 4°C
ARG81900-011 Plate sealer 4 strips Room temperature

Overview

Product Description ARG81900 Mouse IL24 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Mouse IL24 in serum, plasma (heparin, EDTA) and cell culture supernatants.
Tested Reactivity Ms
Tested Application ELISA
Specificity There is no detectable cross-reactivity with other relevant proteins.
Target Name IL24
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm.
Sensitivity 31.25 pg/ml
Sample Type Serum, plasma (heparin, EDTA) and cell culture supernatants.
Standard Range 62.5 - 4000 pg/ml
Sample Volume 100 µl
Precision Intra-Assay CV: 5.2%; Inter-Assay CV: 5.8%
Alternate Names IL10B; Interleukin-24; Melanoma differentiation-associated gene 7 protein; MDA7; MDA-7; C49A; ST16; FISP; Suppression of tumorigenicity 16 protein; IL-24; MOB5

Application Instructions

Assay Time ~ 5 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 93672 Mouse IL24

Swiss-port # Q925S4 Mouse Interleukin-24

Gene Symbol IL24
Gene Full Name interleukin 24
Background This gene encodes a member of the IL10 family of cytokines. It was identified as a gene induced during terminal differentiation in melanoma cells. The protein encoded by this gene can induce apoptosis selectively in various cancer cells. Overexpression of this gene leads to elevated expression of several GADD family genes, which correlates with the induction of apoptosis. The phosphorylation of mitogen-activated protein kinase 14 (MAPK7/P38), and heat shock 27kDa protein 1 (HSPB2/HSP27) are found to be induced by this gene in melanoma cells, but not in normal immortal melanocytes. Alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008]
Function Has antiproliferative properties on melanoma cells and may contribute to terminal cell differentiation. [UniProt]
Highlight Related products:
IL24 antibodies; IL24 ELISA Kits; IL24 recombinant proteins;
New ELISA data calculation tool:
Simplify the ELISA analysis by GainData
PTM Ubiquitination at Lys-122 promotes proteasomal degradation. [UniProt]
Title Download Link
ARG81900 Mouse IL24 ELISA Kit User's manual download icon Download