ARG70175
Mouse IL25 / IL17E recombinant protein (Active) (His-tagged, C-ter)
Mouse IL25 / IL17E recombinant protein (Active) (His-tagged, C-ter) for SDS-PAGE
Overview
| Product Description | E. coli expressed, His-tagged (C-ter) Active Mouse IL25 / IL17E recombinant protein |
|---|---|
| Tested Application | SDS-PAGE |
| Target Name | IL25 / IL17E |
| Species | Mouse |
| A.A. Sequence | MVSLR IQEGC SHLPS CCPSK EQEPP EEWLK WSSAS VSPPE PLSHT HHAES CRASK DGPLN SRAIS PWSYE LDRDL NRVPQ DLYHA RCLCP HCVSL QTGSH MDPLG NSVPL YHNQT VFYRR PCHGE EGTHR RYCLE RRLYR VSLAC VCVRP RVMA with polyhistidine tag at the C-terminus. |
| Expression System | E. coli |
| Activity | Active |
| Activity Note | Measured by its ability to induce CXCL1 secretion in HT‑29 cells. The ED50 for this effect is < 1 ng/ml. |
| Alternate Names | IL17E; Interleukin-17E; IL-25; Interleukin-25; IL-17E |
Properties
| Form | Powder |
|---|---|
| Purification | Ni-NTA chromatography. |
| Purification Note | Endotoxin level is less than 0.1 EU/µg of the protein, as determined by the LAL test. |
| Purity | > 98% (by SDS-PAGE) |
| Buffer | 20 mM Sodium citrate (pH 4.5) and 0.2 M NaCl. |
| Reconstitution | It is recommended to reconstitute the lyophilized protein in sterile water to a concentration not less than 100 µg/ml and incubate the stock solution for at least 20 min at room temperature to make sure the protein is dissolved completely. |
| Storage Instruction | Lyophilized protein should be stored at -20°C. After reconstitution, aliquot and store at -20°C or -80°C for up to one month. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Gene Symbol | IL25 |
|---|---|
| Gene Full Name | interleukin 25 |
| Background | The protein encoded by this gene is a cytokine that shares sequence similarity with interleukin 17. This cytokine can induce NF-kappaB activation, and stimulate the production of interleukin 8. Both this cytokine and interleukin 17B are ligands for the cytokine receptor IL17BR. Studies of a similar gene in mice suggest that this cytokine may be a pro-inflammatory cytokine favoring the Th2-type immune response. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2010] |
| Function | Induces activation of NF-kappa-B and stimulates production of the proinflammatory chemokine IL-8. Proinflammatory cytokine favoring Th2-type immune responses. [UniProt] |
| Cellular Localization | Secreted. [UniProt] |
