ARG80214

Mouse IL27 ELISA Kit

Mouse IL27 ELISA Kit for ELISA and Mouse

Cancer kit; Immune System kit

Component

Cat No Component Name Package Temp
ARG80214-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG80214-002 Standard (Lyophilized) 2 X 2 ng/vial 4°C
ARG80214-003 Standard diluent buffer 20 ml 4°C
ARG80214-004 Antibody conjugate concentrate 400 µl 4°C
ARG80214-005 Antibody diluent buffer 16 ml 4°C
ARG80214-006 HRP-Streptavidin concentrate 400 µl 4°C (Protect from light)
ARG80214-007 HRP-Streptavidin diluent buffer 16 ml 4°C
ARG80214-008 20X Wash buffer 50 ml 4°C
ARG80214-009 TMB substrate 12ml 4°C (Protect from light)
ARG80214-010 STOP solution 12ml 4°C
ARG80214-011 Plate sealer 4 strips Room temperature

Overview

Product Description ARG80214 Mouse IL27 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Mouse IL27 in Serum, Plasma, Cell culture supernatants.
Tested Reactivity Ms
Tested Application ELISA
Specificity No significant cross-reactivity or interference with Mouse IL-6, IL-6 R IL-12 p70, IL-12 p35, IL-12 p40, IL-23, IL-23 p19, TCCR/Fc Chimera;human IL-12/IL-23 p40
Target Name IL27
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm
Sensitivity 30 pg/ml
Sample Type Serum, Plasma, Cell culture supernatants
Standard Range 62.5 - 4000 pg/ml
Sample Volume 100 µl
Precision CV: <10%
Alternate Names IL-27-A; IL27-A; IL-27; Interleukin-27 subunit alpha; IL-27 subunit alpha; IL27A; IL30; Interleukin-30; IL-27A; p28; IL27p28

Application Instructions

Assay Time 4 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 246779 Mouse IL27

Swiss-port # Q8K3I6 Mouse Interleukin-27 subunit alpha

Gene Symbol Il27
Gene Full Name interleukin 27
Background The protein encoded by this gene is one of the subunits of a heterodimeric cytokine complex. This protein is related to interleukin 12A (IL12A). It interacts with Epstein-Barr virus induced gene 3 (EBI3), a protein similar to interleukin 12B (IL12B), and forms a complex that has been shown to drive rapid expansion of naive but not memory CD4(+) T cells. The complex is also found to synergize strongly with interleukin 12 to trigger interferon gamma (IFNG) production of naive CD4(+) T cells. The biological effects of this cytokine are mediated by the class I cytokine receptor (WSX1/TCRR). [provided by RefSeq]
Highlight Related products:
IL27 antibodies; IL27 ELISA Kits; IL27 recombinant proteins;
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Simplify the ELISA analysis by GainData
Research Area Cancer kit; Immune System kit
PTM O-glycosylated.
Title Download Link
ARG80214 Mouse IL27 ELISA Kit User's manual download icon Download