ARG70176
Mouse IL27B / EBI3 recombinant protein (Active) (His-tagged, C-ter)
Mouse IL27B / EBI3 recombinant protein (Active) (His-tagged, C-ter) for SDS-PAGE
Overview
| Product Description | E. coli expressed, His-tagged (C-ter) Active Mouse IL27B / EBI3 recombinant protein |
|---|---|
| Tested Application | SDS-PAGE |
| Target Name | IL27B / EBI3 |
| Species | Mouse |
| A.A. Sequence | MALVA LSQPR VQCHA SRYPV AVDCS WTPLQ APNST RSTSF IATYR LGVAT QQQSQ PCLQR SPQAS RCTIP DVHLF STVPY MLNVT AVHPG GASSS LLAFV AERII KPDPP EGVRL RTAGQ RLQVL WHPPA SWPFP DIFSL KYRLR YRRRG ASHFR QVGPI EATTF TLRNS KPHAK YCIQV SAQDL TDYGK PSDWS LPGQV ESAPH KP with polyhistidine tag at the C-terminus. |
| Expression System | E. coli |
| Activity | Active |
| Activity Note | Determined by its ability to protect HepG2 cells infected with encephalomyocarditis (EMC) virus. The ED50 for this effect is < 5 ng/ml. |
| Alternate Names | EBV-induced gene 3 protein; IL-27 subunit beta; IL27B; Epstein-Barr virus-induced gene 3 protein; Interleukin-27 subunit beta; IL-27B |
Properties
| Form | Powder |
|---|---|
| Purification | Ni-NTA chromatography. |
| Purification Note | Endotoxin level is less than 0.1 EU/µg of the protein, as determined by the LAL test. |
| Purity | > 95% (by SDS-PAGE) |
| Buffer | 20 mM Sodium carbonate (pH 4.5) and 0.2M NaCl. |
| Reconstitution | It is recommended to reconstitute the lyophilized protein in sterile water to a concentration not less than 100 µg/ml and incubate the stock solution for at least 20 min at room temperature to make sure the protein is dissolved completely. |
| Storage Instruction | Lyophilized protein should be stored at -20°C. After reconstitution, aliquot and store at -20°C or -80°C for up to one month. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Gene Symbol | EBI3 |
|---|---|
| Gene Full Name | Epstein-Barr virus induced 3 |
| Background | This gene was identified by its induced expression in B lymphocytes in response Epstein-Barr virus infection. It encodes a secreted glycoprotein belonging to the hematopoietin receptor family, and heterodimerizes with a 28 kDa protein to form interleukin 27 (IL-27). IL-27 regulates T cell and inflammatory responses, in part by activating the Jak/STAT pathway of CD4+ T cells. [provided by RefSeq, Sep 2008] |
| Function | Associates with IL27 to form the IL-27 interleukin, a heterodimeric cytokine which functions in innate immunity. IL-27 has pro- and anti-inflammatory properties, that can regulate T-helper cell development, suppress T-cell proliferation, stimulate cytotoxic T-cell activity, induce isotype switching in B-cells, and that has diverse effects on innate immune cells. Among its target cells are CD4 T-helper cells which can differentiate in type 1 effector cells (TH1), type 2 effector cells (TH2) and IL17 producing helper T-cells (TH17). It drives rapid clonal expansion of naive but not memory CD4 T-cells. It also strongly synergizes with IL-12 to trigger interferon-gamma/IFN-gamma production of naive CD4 T-cells, binds to the cytokine receptor WSX-1/TCCR. Another important role of IL-27 is its antitumor activity as well as its antiangiogenic activity with activation of production of antiangiogenic chemokines. [UniProt] |
| Cellular Localization | Secreted. [UniProt] |
