ARG70155
Mouse IL3 recombinant protein (Active) (His-tagged, C-ter)
Mouse IL3 recombinant protein (Active) (His-tagged, C-ter) for SDS-PAGE
Overview
| Product Description | E. coli expressed, His-tagged (C-ter) Active Mouse IL3 recombinant protein |
|---|---|
| Tested Application | SDS-PAGE |
| Target Name | IL3 |
| Species | Mouse |
| A.A. Sequence | MDTHR LTRTL NCSSI VKEII GKLPE PELKT DDEGP SLRNK SFRRV NLSKF VESQG EVDPE DRYVI KSNLQ KLNCC LPTSA NDSAL PGVFI RDLDD FRKKL RFYMV HLNDL ETVLT SRPPQ PASGS VSPNR GTVEC with polyhistidine tag at the C-terminus. |
| Expression System | E. coli |
| Activity | Active |
| Activity Note | Determined by its ability to induce NFS-60 cells proliferation. The ED50 for this effect is < 85 pg/ml. The specific activity of recombinant mouse IL-3 is approximately > 1x 10^7 IU/mg. |
| Alternate Names | MCGF; Mast cell growth factor; P-cell-stimulating factor; Hematopoietic growth factor; IL-3; Interleukin-3; Multipotential colony-stimulating factor; MULTI-CSF |
Properties
| Form | Powder |
|---|---|
| Purification | Ni-NTA chromatography. |
| Purification Note | Endotoxin level is less than 0.1 EU/µg of the protein, as determined by the LAL test. |
| Purity | > 98% (by SDS-PAGE) |
| Buffer | PBS (pH 7.4) |
| Reconstitution | It is recommended to reconstitute the lyophilized protein in sterile water to a concentration not less than 100 µg/ml and incubate the stock solution for at least 20 min at room temperature to make sure the protein is dissolved completely. |
| Storage Instruction | Lyophilized protein should be stored at -20°C. After reconstitution, aliquot and store at -20°C or -80°C for up to one month. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Gene Symbol | IL3 |
|---|---|
| Gene Full Name | interleukin 3 |
| Background | The protein encoded by this gene is a potent growth promoting cytokine. This cytokine is capable of supporting the proliferation of a broad range of hematopoietic cell types. It is involved in a variety of cell activities such as cell growth, differentiation and apoptosis. This cytokine has been shown to also possess neurotrophic activity, and it may be associated with neurologic disorders. [provided by RefSeq, Jul 2008] |
| Function | Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. This CSF induces granulocytes, macrophages, mast cells, stem cells, erythroid cells, eosinophils and megakaryocytes. [UniProt] |
| Cellular Localization | Secreted. [UniProt] |
