ARG70181
Mouse IL34 recombinant protein (Active) (His-tagged, C-ter)
Mouse IL34 recombinant protein (Active) (His-tagged, C-ter) for SDS-PAGE
Overview
| Product Description | E. coli expressed, His-tagged (C-ter) Active Mouse IL34 recombinant protein |
|---|---|
| Tested Application | SDS-PAGE |
| Target Name | IL34 |
| Species | Mouse |
| A.A. Sequence | MNENL EIWTL TQDKE CDLTG YLRGK LQYKN RLQYM KHYFP INYRI AVPYE GVLRV ANITR LQKAH VSERE LRYLW VLVSL NATES VMDVL LEGHP SWKYL QEVQT LLENV QRSLM DVEIG PHVEA VLSLL STPGL SLKLV RPKAL LDNCF RVMEL LYCSC CKQSP ILKWQ DCELP RLHPH SPGSL MQCTA TNVYP LSRQT PTSLP GSPSS SHGSL P with polyhistidine tag at the C-terminus. |
| Expression System | E. coli |
| Activity | Active |
| Activity Note | Determined by its ability to induce proliferation in NFS-60 cells. The ED50 for this effect is < 30 ng/ml. The specific activity of recombinant mouse IL-34 is > 3.3 x 10^4 IU/mg. |
| Alternate Names | IL-34; Interleukin-34; C16orf77 |
Properties
| Form | Powder |
|---|---|
| Purification | Ni-NTA chromatography. |
| Purification Note | Endotoxin level is less than 0.1 EU/µg of the protein, as determined by the LAL test. |
| Purity | > 98% (by SDS-PAGE) |
| Buffer | PBS (pH 7.4) |
| Reconstitution | It is recommended to reconstitute the lyophilized protein in sterile water to a concentration not less than 100 µg/ml and incubate the stock solution for at least 20 min at room temperature to make sure the protein is dissolved completely. |
| Storage Instruction | Lyophilized protein should be stored at -20°C. After reconstitution, aliquot and store at -20°C or -80°C for up to one month. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Gene Symbol | IL34 |
|---|---|
| Gene Full Name | interleukin 34 |
| Background | Interleukin-34 is a cytokine that promotes the differentiation and viability of monocytes and macrophages through the colony-stimulating factor-1 receptor (CSF1R; MIM 164770) (Lin et al., 2008 [PubMed 18467591]).[supplied by OMIM, May 2008] |
| Function | Cytokine that promotes the proliferation, survival and differentiation of monocytes and macrophages. Promotes the release of proinflammatory chemokines, and thereby plays an important role in innate immunity and in inflammatory processes. Plays an important role in the regulation of osteoclast proliferation and differentiation, and in the regulation of bone resorption. Signaling via CSF1R and its downstream effectors stimulates phosphorylation of MAPK1/ERK2 AND MAPK3/ERK1. [UniProt] |
| Cellular Localization | Secreted. [UniProt] |
