ARG70157
Mouse IL5 recombinant protein (Active) (His-tagged, C-ter)
Mouse IL5 recombinant protein (Active) (His-tagged, C-ter) for SDS-PAGE
Overview
Product Description | E. coli expressed, His-tagged (C-ter) Active Mouse IL5 recombinant protein |
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Tested Application | SDS-PAGE |
Target Name | IL5 |
Species | Mouse |
A.A. Sequence | MEIPM STVVK ETLTQ LSAHR ALLTS NETMR LPVPT HKNHQ LCIGE IFQGL DILKN QTVRG GTVEM LFQNL SLIKK YIDRQ KEKCG EERRR TRQFL DYLQE FLGVM STEWA MEG with polyhistidine tag at the C-terminus. |
Expression System | E. coli |
Activity | Active |
Activity Note | Determined by its ability to induce TF-1 cells proliferation. The ED50 for this effect is < 0.2 ng/ml. The specific activity of recombinant mouse IL-5 is > 5 x 10^6 IU/mg. |
Alternate Names | Eosinophil differentiation factor; EDF; IL-5; TRF; T-cell replacing factor; B-cell differentiation factor I; Interleukin-5 |
Properties
Form | Powder |
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Purification | Ni-NTA chromatography. |
Purification Note | Endotoxin level is less than 0.1 EU/µg of the protein, as determined by the LAL test. |
Purity | > 98% (by SDS-PAGE) |
Buffer | PBS (pH 7.4) |
Reconstitution | It is recommended to reconstitute the lyophilized protein in sterile water to a concentration not less than 100 µg/ml and incubate the stock solution for at least 20 min at room temperature to make sure the protein is dissolved completely. |
Storage Instruction | Lyophilized protein should be stored at -20°C. After reconstitution, aliquot and store at -20°C or -80°C for up to one month. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. |
Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
Gene Symbol | IL5 |
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Gene Full Name | interleukin 5 |
Background | This gene encodes a cytokine that acts as a growth and differentiation factor for both B cells and eosinophils. The encoded cytokine plays a major role in the regulation of eosinophil formation, maturation, recruitment and survival. The increased production of this cytokine may be related to pathogenesis of eosinophil-dependent inflammatory diseases. This cytokine functions by binding to its receptor, which is a heterodimer, whose beta subunit is shared with the receptors for interleukine 3 (IL3) and colony stimulating factor 2 (CSF2/GM-CSF). This gene is located on chromosome 5 within a cytokine gene cluster which includes interleukin 4 (IL4), interleukin 13 (IL13), and CSF2 . This gene, IL4, and IL13 may be regulated coordinately by long-range regulatory elements spread over 120 kilobases on chromosome 5q31. [provided by RefSeq, Jul 2013] |
Function | Factor that induces terminal differentiation of late-developing B-cells to immunoglobulin secreting cells. [UniProt] |
Cellular Localization | Secreted. [UniProt] |