ARG70160

Mouse IL9 recombinant protein (Active) (His-tagged, N-ter)

Mouse IL9 recombinant protein (Active) (His-tagged, N-ter) for SDS-PAGE

Overview

Product Description E. coli expressed, His-tagged (N-ter) Active Mouse IL9 recombinant protein
Tested Application SDS-PAGE
Target Name IL9
Species Mouse
A.A. Sequence QRCST TWGIR DTNYL IENLK DDPPS KCSCS GNVTS CLCLS VPTDD CTTPC YRE G LLQLT NATQK SRLLP VFHRV KRIVE VLKNI TCPSF SCEKP CNQTM AGNTL SFLK SLLGT FQKTE MQRQK SRP with polyhistidine tag at the N-terminus.
Expression System E. coli
Activity Active
Activity Note Determined by its ability to induce proliferation in MO7e cells. The ED50 for this effect is < 0.2 ng/ml. The specific activity of recombinant mouse IL-9 is > 5 x 10^6 IU/mg.
Alternate Names IL-9; HP40; Interleukin-9; T-cell growth factor P40; Cytokine P40; P40

Properties

Form Powder
Purification Ni-NTA chromatography.
Purification Note Endotoxin level is less than 0.1 EU/µg of the protein, as determined by the LAL test.
Purity > 98% (by SDS-PAGE)
Buffer PBS (pH 7.4)
Reconstitution It is recommended to reconstitute the lyophilized protein in sterile water to a concentration not less than 100 µg/ml and incubate the stock solution for at least 20 min at room temperature to make sure the protein is dissolved completely.
Storage Instruction Lyophilized protein should be stored at -20°C. After reconstitution, aliquot and store at -20°C or -80°C for up to one month. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Gene Symbol IL9
Gene Full Name interleukin 9
Background The protein encoded by this gene is a cytokine that acts as a regulator of a variety of hematopoietic cells. This cytokine stimulates cell proliferation and prevents apoptosis. It functions through the interleukin 9 receptor (IL9R), which activates different signal transducer and activator (STAT) proteins and thus connects this cytokine to various biological processes. The gene encoding this cytokine has been identified as a candidate gene for asthma. Genetic studies on a mouse model of asthma demonstrated that this cytokine is a determining factor in the pathogenesis of bronchial hyperresponsiveness. [provided by RefSeq, Jul 2008]
Function Supports IL-2 independent and IL-4 independent growth of helper T-cells. [UniProt]
Cellular Localization Secreted. [UniProt]