ARG42252

Mouse IgG2a Isotype Control antibody [MOPC-173] (Biotin)

Mouse IgG2a Isotype Control antibody [MOPC-173] (Biotin) for Control,Flow cytometry

Overview

Product Description Biotin-conjugated Mouse Monoclonal antibody [MOPC-173] as a negative control antibody for Mouse IgG2a
Species Does Not React With Hu, Ms, Rat
Tested Application Control, FACS
Specificity This mouse IgG2a monoclonal antibody (clone MOPC-173) reacts with an unknown epitope. It does not react with a variety of resting, activated, live, and fixed mouse, rat and human tissues.
Host Mouse
Clonality Monoclonal
Clone MOPC-173
Isotype IgG2a, kappa
Target Name Mouse IgG2a
Immunogen Induced by intraperitoneal injection of mineral oils into BALB/c mice.
Conjugation Biotin

Application Instructions

Application Suggestion
Tested Application Dilution
ControlAssay-dependent
FACSAssay-dependent
Application Note Negative control: The reagent is intended as an isotype control to establish the amount of non-specific antibody binding. For your particular experiment, use the same concentration of this control antibody as the recommended working concentration of the antigen-specific antibody. Also, when working with prediluted antibodies, dilute the isotype control to the same concentration as is the concentration of the antigen-specific antibody in the prediluted antibody solution you are using. If under particular experimental conditions the background signal of the isotype control is too high (usually when working concentrations of used antibodies are above 10 μg/ml of incubation mixture), change the conditions of your experiment to reduce the background.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purified
Buffer PBS and 15 mM Sodium azide.
Preservative 15 mM Sodium azide
Concentration 1 mg/ml
Storage Instruction Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.