ARG65325
Mouse IgG2b Isotype Control antibody [MPC-11]
Mouse IgG2b Isotype Control antibody [MPC-11] for Control,ELISA,Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,IHC-Frozen sections,Immunoprecipitation,Western blot
Controls and Markers antibody
Overview
| Product Description | Mouse Monoclonal antibody [MPC-11] as a negative control antibody for Mouse IgG2b |
|---|---|
| Species Does Not React With | Hu, Ms, Rat |
| Tested Application | Control, ELISA, FACS, ICC/IF, IHC-Fr, IHC-P, IP, WB |
| Specificity | This mouse IgG2b kappa monoclonal antibody (clone MPC-11) reacts with an epitope irrelevant for a variety of resting, activated, live, and fixed human, mouse, and rat tissues. |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | MPC-11 |
| Isotype | IgG2b |
| Target Name | Mouse IgG2b |
| Immunogen | KLH-coupled trinitrophenol |
| Conjugation | Un-conjugated |
Application Instructions
| Application Suggestion |
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| Application Note | This isotppe control antibody is intended as Mouse IgG2b isotype control for the analysis to establish the amount of non-specific antibody binding. Using the same concentration of this isotype control antibody as the recommended working concentration of the antigen-specific antibody. In addition, when working with prediluted antibodies, dilute the isotype control to the same concentration as is the concentration of the antigen-specific antibody in the prediluted antibody solution using in the experiment. If under particular experimental conditions the background signal of the isotype control is too high (usually when working concentrations of used antibodies are above 10 µg per ml of incubation mixture), change the conditions of your experiment to reduce the background. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from hybridoma culture supernatant by protein-A affinity chromatography. |
| Purity | > 95% (by SDS-PAGE) |
| Buffer | PBS (pH 7.4) and 15 mM Sodium azide |
| Preservative | 15 mM Sodium azide |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Background | The specificity of staining by monoclonal antibodies to target antigens should be verified by establishing the amount of non-specific antibody binding. Especially at higher concentration (more than 15 μg/ml) the antibody staining usually has consignable background. To this end a non-reactive immunoglobulin of the same isotype is included as a negative control for each specific monoclonal antibody used in a particular immunoassay. The monoclonal antibody MPC-11 was generated against an epitope irrelevant for human, mouse, and rat material, and can thus be used for evaluation of the background staining that is caused by general nonspecific interactions between an mouse IgG2b molecule and the respective sample under the particular conditions. This shall help the customer to set up the experimental conditions so that the nonspecific binding of any antibody is abolished. |
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| Research Area | Controls and Markers antibody |
