ARG82354

Mouse KREMEN1 ELISA Kit

Mouse KREMEN1 ELISA Kit for ELISA and Mouse

Component

Cat No Component Name Package Temp
ARG82354-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG82354-002 Standard 2 X 10 ng/vial 4°C
ARG82354-003 Standard/Sample diluent 30 ml (Ready to use) 4°C
ARG82354-004 Antibody conjugate concentrate (100X) 1 vial (100 µl) 4°C
ARG82354-005 Antibody diluent buffer 12 ml (Ready to use) 4°C
ARG82354-006 HRP-Streptavidin concentrate (100X) 1 vial (100 µl) 4°C
ARG82354-007 HRP-Streptavidin diluent buffer 12 ml (Ready to use) 4°C
ARG82354-008 25X Wash buffer 20 ml 4°C
ARG82354-009 TMB substrate 10 ml (Ready to use) 4°C (Protect from light)
ARG82354-010 STOP solution 10 ml (Ready to use) 4°C
ARG82354-011 Plate sealer 4 strips Room temperature

Overview

Product Description ARG82354 Mouse KREMEN1 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Mouse KREMEN1 in serum, plasma (EDTA, heparin) and cell culture supernatants.
Tested Reactivity Ms
Tested Application ELISA
Target Name KREMEN1
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm.
Sensitivity 62.5 pg/ml
Sample Type Serum, plasma (EDTA, heparin) and cell culture supernatants.
Standard Range 125 - 8000 pg/ml
Sample Volume 100 µl
Precision Intra-Assay CV: 6.1%
Inter-Assay CV: 6.7%
Alternate Names KREMEN; KRM1; Dickkopf receptor; Kringle domain-containing transmembrane protein 1; Kremen protein 1; Kringle-containing protein marking the eye and the nose

Application Instructions

Assay Time ~ 5 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 84035 Mouse KREMEN1

Swiss-port # Q99N43 Mouse Kremen protein 1

Gene Symbol KREMEN1
Gene Full Name kringle containing transmembrane protein 1
Background This gene encodes a high-affinity dickkopf homolog 1 (DKK1) transmembrane receptor that functionally cooperates with DKK1 to block wingless (WNT)/beta-catenin signaling. The encoded protein is a component of a membrane complex that modulates canonical WNT signaling through lipoprotein receptor-related protein 6 (LRP6). It contains extracellular kringle, WSC, and CUB domains. Alternatively spliced transcript variants encoding distinct isoforms have been observed for this gene. [provided by RefSeq, Jul 2008]
Function Receptor for Dickkopf protein. Cooperates with Dickkopf to block Wnt/beta-catenin signaling (By similarity). [UniProt]
Cellular Localization Cell membrane; Single-pass type I membrane protein. [UniProt]
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Title Download Link
ARG82354 Mouse KREMEN1 ELISA Kit User manual download icon Download