ARG82080

Mouse Leptin Receptor ELISA Kit

Mouse Leptin Receptor ELISA Kit for ELISA and Mouse

Component

Cat No Component Name Package Temp
ARG82080-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG82080-002 Standard 2 X 10 ng/vial 4°C
ARG82080-003 Standard/Sample diluent 30 ml (Ready to use) 4°C
ARG82080-004 Antibody conjugate concentrate (100X) 1 vial (100 µl) 4°C
ARG82080-005 Antibody diluent buffer 12 ml (Ready to use) 4°C
ARG82080-006 HRP-Streptavidin concentrate (100X) 1 vial (100 µl) 4°C
ARG82080-007 HRP-Streptavidin diluent buffer 12 ml (Ready to use) 4°C
ARG82080-008 25X Wash buffer 20 ml 4°C
ARG82080-009 TMB substrate 10 ml (Ready to use) 4°C (Protect from light)
ARG82080-010 STOP solution 10 ml (Ready to use) 4°C
ARG82080-011 Plate sealer 4 strips Room temperature

Overview

Product Description ARG82080 Mouse Leptin Receptor ELISA Kit is an Enzyme Immunoassay kit for the quantification of Mouse Leptin Receptor in serum, plasma (heparin, EDTA) and cell culture supernatants.
Tested Reactivity Ms
Tested Application ELISA
Specificity There is no detectable cross-reactivity with other relevant proteins.
Target Name Leptin Receptor
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm.
Sensitivity 78 pg/ml
Sample Type Serum, plasma (heparin, EDTA) and cell culture supernatants.
Standard Range 156 - 10000 pg/ml
Sample Volume 100 µl
Precision Intra-Assay CV: 6.7%; Inter-Assay CV: 7.8%
Alternate Names LEPRD; CD antigen CD295; CD295; Leptin receptor; LEP-R; HuB219; OB-R; OBR; OB receptor

Application Instructions

Assay Time ~ 5 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 16847 Mouse LEPR

Swiss-port # P48356 Mouse Leptin receptor

Gene Symbol LEPR
Gene Full Name leptin receptor
Background The protein encoded by this gene belongs to the gp130 family of cytokine receptors that are known to stimulate gene transcription via activation of cytosolic STAT proteins. This protein is a receptor for leptin (an adipocyte-specific hormone that regulates body weight), and is involved in the regulation of fat metabolism, as well as in a novel hematopoietic pathway that is required for normal lymphopoiesis. Mutations in this gene have been associated with obesity and pituitary dysfunction. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. It is noteworthy that this gene and LEPROT gene (GeneID:54741) share the same promoter and the first 2 exons, however, encode distinct proteins (PMID:9207021).[provided by RefSeq, Nov 2010]
Function Receptor for obesity factor (leptin). On ligand binding, mediates signaling through JAK2/STAT3. Involved in the regulation of fat metabolism and, in a hematopoietic pathway, required for normal lymphopoiesis. May play a role in reproduction. Can also mediate the ERK/FOS signaling pathway (By similarity). [UniProt]
Cellular Localization Cell membrane; Single-pass type I membrane protein. Basolateral cell membrane. Isoform E: Secreted. [UniProt]
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PTM On ligand binding, phosphorylated on two conserved C-terminal tyrosine residues (isoform B only) by JAK2. Tyr-986 is required for complete binding and activation of PTPN11, ERK/FOS activation,for interaction with SOCS3 and SOCS3 mediated inhibition of leptin signaling. Phosphorylation on Tyr-1141 is required for STAT3 binding/activation. Phosphorylation of Tyr-1079 has a more accessory role. [UniProt]
Title Download Link
ARG82080 Mouse Leptin Receptor ELISA Kit User's manual download icon Download