ARG82080
Mouse Leptin Receptor ELISA Kit
Mouse Leptin Receptor ELISA Kit for ELISA and Mouse
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG82080-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG82080-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG82080-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG82080-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82080-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82080-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82080-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82080-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG82080-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG82080-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG82080-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG82080 Mouse Leptin Receptor ELISA Kit is an Enzyme Immunoassay kit for the quantification of Mouse Leptin Receptor in serum, plasma (heparin, EDTA) and cell culture supernatants. |
|---|---|
| Tested Reactivity | Ms |
| Tested Application | ELISA |
| Specificity | There is no detectable cross-reactivity with other relevant proteins. |
| Target Name | Leptin Receptor |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 78 pg/ml |
| Sample Type | Serum, plasma (heparin, EDTA) and cell culture supernatants. |
| Standard Range | 156 - 10000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 6.7%; Inter-Assay CV: 7.8% |
| Alternate Names | LEPRD; CD antigen CD295; CD295; Leptin receptor; LEP-R; HuB219; OB-R; OBR; OB receptor |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | LEPR |
| Gene Full Name | leptin receptor |
| Background | The protein encoded by this gene belongs to the gp130 family of cytokine receptors that are known to stimulate gene transcription via activation of cytosolic STAT proteins. This protein is a receptor for leptin (an adipocyte-specific hormone that regulates body weight), and is involved in the regulation of fat metabolism, as well as in a novel hematopoietic pathway that is required for normal lymphopoiesis. Mutations in this gene have been associated with obesity and pituitary dysfunction. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. It is noteworthy that this gene and LEPROT gene (GeneID:54741) share the same promoter and the first 2 exons, however, encode distinct proteins (PMID:9207021).[provided by RefSeq, Nov 2010] |
| Function | Receptor for obesity factor (leptin). On ligand binding, mediates signaling through JAK2/STAT3. Involved in the regulation of fat metabolism and, in a hematopoietic pathway, required for normal lymphopoiesis. May play a role in reproduction. Can also mediate the ERK/FOS signaling pathway (By similarity). [UniProt] |
| Cellular Localization | Cell membrane; Single-pass type I membrane protein. Basolateral cell membrane. Isoform E: Secreted. [UniProt] |
| Highlight | Related products: Leptin Receptor antibodies; Leptin Receptor ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| PTM | On ligand binding, phosphorylated on two conserved C-terminal tyrosine residues (isoform B only) by JAK2. Tyr-986 is required for complete binding and activation of PTPN11, ERK/FOS activation,for interaction with SOCS3 and SOCS3 mediated inhibition of leptin signaling. Phosphorylation on Tyr-1141 is required for STAT3 binding/activation. Phosphorylation of Tyr-1079 has a more accessory role. [UniProt] |
| Title | Download Link |
|---|---|
| ARG82080 Mouse Leptin Receptor ELISA Kit User's manual |
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