ARG81764

Mouse Lipocalin 2 ELISA Kit

Mouse Lipocalin 2 ELISA Kit for ELISA and Mouse

Component

Cat No Component Name Package Temp
ARG81764-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG81764-002 Standard 2 X 10 ng/vial 4°C
ARG81764-003 Standard/Sample diluent 30 ml (Ready to use) 4°C
ARG81764-004 Antibody conjugate concentrate (100X) 1 vial (100 µl) 4°C
ARG81764-005 Antibody diluent buffer 12 ml (Ready to use) 4°C
ARG81764-006 HRP-Streptavidin concentrate (100X) 1 vial (100 µl) 4°C
ARG81764-007 HRP-Streptavidin diluent buffer 12 ml (Ready to use) 4°C
ARG81764-008 25X Wash buffer 20 ml 4°C
ARG81764-009 TMB substrate 10 ml (Ready to use) 4°C (Protect from light)
ARG81764-010 STOP solution 10 ml (Ready to use) 4°C
ARG81764-011 Plate sealer 4 strips Room temperature

Overview

Product Description ARG81764 Mouse Lipocalin 2 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Mouse Lipocalin 2 in serum, plasma (heparin), urine and cell culture supernatants.
Tested Reactivity Ms
Tested Application ELISA
Specificity There is no detectable cross-reactivity with other relevant proteins.
Target Name Lipocalin 2
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm.
Sensitivity 39 pg/ml
Sample Type Serum, plasma (heparin), urine and cell culture supernatants.
Standard Range 78 - 5000 pg/ml
Sample Volume 100 µl
Precision Intra-Assay CV: 4.6%; Inter-Assay CV: 6.2%
Alternate Names Siderocalin LCN2; Oncogene 24p3; MSFI; 25 kDa alpha-2-microglobulin-related subunit of MMP-9; Lipocalin-2; p25; Neutrophil gelatinase-associated lipocalin; 24p3; NGAL

Application Instructions

Assay Time ~ 5 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 16819 Mouse LCN2

Swiss-port # P11672 Mouse Neutrophil gelatinase-associated lipocalin

Gene Symbol LCN2
Gene Full Name lipocalin 2
Background This gene encodes a protein that belongs to the lipocalin family. Members of this family transport small hydrophobic molecules such as lipids, steroid hormones and retinoids. The protein encoded by this gene is a neutrophil gelatinase-associated lipocalin and plays a role in innate immunity by limiting bacterial growth as a result of sequestering iron-containing siderophores. The presence of this protein in blood and urine is an early biomarker of acute kidney injury. This protein is thought to be be involved in multiple cellular processes, including maintenance of skin homeostasis, and suppression of invasiveness and metastasis. Mice lacking this gene are more susceptible to bacterial infection than wild type mice. [provided by RefSeq, Sep 2015]
Function Iron-trafficking protein involved in multiple processes such as apoptosis, innate immunity and renal development. Binds iron through association with 2,5-dihydroxybenzoic acid (2,5-DHBA), a siderophore that shares structural similarities with bacterial enterobactin, and delivers or removes iron from the cell, depending on the context. Iron-bound form (holo-24p3) is internalized following binding to the SLC22A17 (24p3R) receptor, leading to release of iron and subsequent increase of intracellular iron concentration. In contrast, association of the iron-free form (apo-24p3) with the SLC22A17 (24p3R) receptor is followed by association with an intracellular siderophore, iron chelation and iron transfer to the extracellular medium, thereby reducing intracellular iron concentration. Involved in apoptosis due to interleukin-3 (IL3) deprivation: iron-loaded form increases intracellular iron concentration without promoting apoptosis, while iron-free form decreases intracellular iron levels, inducing expression of the proapoptotic protein BCL2L11/BIM, resulting in apoptosis. Involved in innate immunity, possibly by sequestrating iron, leading to limit bacterial growth. [UniProt]
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Title Download Link
ARG81764 Mouse Lipocalin 2 ELISA Kit User's manual download icon Download