ARG70193

Mouse M-CSF recombinant protein (Active) (His-tagged, C-ter)

Mouse M-CSF recombinant protein (Active) (His-tagged, C-ter) for SDS-PAGE

Overview

Product Description E. coli expressed, His-tagged (C-ter) Active Mouse M-CSF recombinant protein
Tested Application SDS-PAGE
Target Name M-CSF
Species Mouse
A.A. Sequence MKEVS EHCSH MIGNG HLKVL QQLID SQMET SCQIA FEFVD QEQLD DPVCY LKKAF FLVQD IIDET MRFKD NTPNA NATER LQELS NNLNS CFTKD YEEQN KACVR TFHET PLQLL EKIKN FFDET KNLLE KDWNI FTKNC NNSFA KCSSR DVVTK P with polyhistidine tag at the C-terminus.
Expression System E. coli
Activity Active
Activity Note Determined by its ability to induce proliferation in NFS-60 cells. The ED50 for this effect is < 2 ng/ml. The specific activity of recombinant mouse M-CSF is approximately > 5x 10^5 IU/mg.
Alternate Names Macrophage colony-stimulating factor 1; CSF-1; Lanimostim; M-CSF; MCSF

Properties

Form Powder
Purification Ni-NTA chromatography.
Purification Note Endotoxin level is less than 0.1 EU/µg of the protein, as determined by the LAL test.
Purity > 98% (by SDS-PAGE)
Buffer PBS (pH 8.0)
Reconstitution It is recommended to reconstitute the lyophilized protein in sterile water to a concentration not less than 100 µg/ml and incubate the stock solution for at least 20 min at room temperature to make sure the protein is dissolved completely.
Storage Instruction Lyophilized protein should be stored at -20°C. After reconstitution, aliquot and store at -20°C or -80°C for up to one month. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Gene Symbol CSF1
Gene Full Name colony stimulating factor 1 (macrophage)
Background The protein encoded by this gene is a cytokine that controls the production, differentiation, and function of macrophages. The active form of the protein is found extracellularly as a disulfide-linked homodimer, and is thought to be produced by proteolytic cleavage of membrane-bound precursors. The encoded protein may be involved in development of the placenta. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Sep 2011]
Function Cytokine that plays an essential role in the regulation of survival, proliferation and differentiation of hematopoietic precursor cells, especially mononuclear phagocytes, such as macrophages and monocytes. Promotes the release of proinflammatory chemokines, and thereby plays an important role in innate immunity and in inflammatory processes. Plays an important role in the regulation of osteoclast proliferation and differentiation, the regulation of bone resorption, and is required for normal bone development. Required for normal male and female fertility. Promotes reorganization of the actin cytoskeleton, regulates formation of membrane ruffles, cell adhesion and cell migration. Plays a role in lipoprotein clearance. [UniProt]
Cellular Localization Cell membrane; Single-pass type I membrane protein. Processed macrophage colony-stimulating factor 1: Secreted, extracellular space. [UniProt]
PTM N- and O-glycosylated. Glycosylation and proteolytic cleavage yield different soluble forms. One high molecular weight soluble form is a proteoglycan containing chondroitin sulfate. O-glycosylated with core 1 or possibly core 8 glycans. Isoform 1 is N- and O-glycosylated. Isoform 3 is only N-glycosylated. [UniProt]