ARG83440
Mouse MEP1B / Meprin Beta ELISA Kit
Mouse MEP1B / Meprin Beta ELISA Kit for ELISA and Mouse
Overview
| Product Description | ARG83440 Mouse MEP1B / Meprin Beta ELISA Kit is an Enzyme Immunoassay kit for the quantification of Mouse MEP1B / Meprin Beta in Serum, plasma and cell culture supernatants., Cell Lysates, Tissue Homogenates |
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| Tested Reactivity | Ms |
| Tested Application | ELISA |
| Target Name | MEP1B / Meprin Beta |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 10 pg/ml |
| Sample Type | Serum, plasma and cell culture supernatants., Cell Lysates, Tissue Homogenates |
| Standard Range | 156 - 10000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: less than 10% Inter-Assay CV: less than 10% |
| Alternate Names | MEP1B; Meprin A Subunit Beta; N-Benzoyl-L-Tyrosyl-P-Amino-Benzoic Acid Hydrolase Subunit Beta; PABA Peptide Hydrolase; Endopeptidase-2; Meprin A, Beta; PPH Beta; Meprin B; N-Benzoyl-L-Tyrosyl-P-Amino-Benzoic Acid Hydrolase Beta Subunit; EC 3.4.24.63 |
Application Instructions
| Assay Time | 3.5 hours |
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Properties
| Form | 96 well |
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| Storage Instruction | Store the kit at 4°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | MEP1B |
| Gene Full Name | Meprin A Subunit Beta |
| Background | Meprins are multidomain zinc metalloproteases that are highly expressed in mammalian kidney and intestinal brush border membranes, and in leukocytes and certain cancer cells. They are involved in the hydrolysis of a variety of peptide and protein substrates, and have been implicated in cancer and intestinal inflammation. Mature meprins are oligomers of evolutionarily related, but separately encoded alpha and/or beta subunits. Homooligomers of alpha subunit are secreted, whereas, oligomers containing the beta subunit are plasma membrane-bound. This gene encodes the beta subunit. Targeted disruption of this gene in mice affects embryonic viability, renal gene expression profiles, and distribution of the membrane-associated alpha subunit in kidney and intestine. |
| Function | Membrane metallopeptidase that sheds many membrane-bound proteins. Exhibits a strong preference for acidic amino acids at the P1' position. Known substrates include: FGF19, VGFA, IL1B, IL18, procollagen I and III, E-cadherin, KLK7, gastrin, ADAM10, tenascin-C. The presence of several pro-inflammatory cytokine among substrates implicate MEP1B in inflammation. It is also involved in tissue remodeling due to its capability to degrade extracellular matrix components. |
| Cellular Localization | Cell membrane, Membrane, Secreted |
| PTM | Disulfide bond, Glycoprotein, Zymogen |
| Title | Download Link |
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| ARG83440 Mouse MEP1B / Meprin Beta ELISA Kit User manual |
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