ARG82074

Mouse MMP1 ELISA Kit

Mouse MMP1 ELISA Kit for ELISA and Mouse

Overview

Product Description ARG82074 Mouse MMP1 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Mouse MMP1 in serum, plasma and cell culture supernatants.
Tested Reactivity Ms
Tested Application ELISA
Specificity The following recombinant Mouse proteins were tested and exhibited no cross-reactivity or interference: Adiponectin, ApoAI, BMP1, BMP2, BMP3, BMP4, BMP5, BMP6, BMP7, CCL2, CCL4, CCL5, CRP, FGF acidic, HGF, HSP27, IGF1, IL1 alpha, IL1 beta, IL1RA, IL4, IL5, IL6, IL8, IL10, IL12, IL13, IL15, IL17C, MMP11, MMP13, IFN alpha, IFN beta, IFN gamma, MMP2, MMP3, MMP9, PDGF, PLA2G7, prolactin, TLR1, TLR2, TLR3, TLR4, TLR9, TGF beta 1, TGF beta 2, TGF beta 3, TNF alpha, TNF RI, TNF RII, VEGF, VEGF R1.
Target Name MMP1
Conjugation Note Read at 450 nm.
Sensitivity 18 pg/ml
Sample Type Serum, plasma and cell culture supernatants.
Standard Range 93 - 6000 pg/ml
Sample Volume 100 µl
Precision Intra-Assay CV: 5%; Inter-Assay CV: 10%
Alternate Names MMP-1; CLG; Fibroblast collagenase; Matrix metalloproteinase-1; CLGN; EC 3.4.24.7; Interstitial collagenase

Application Instructions

Assay Time ~ 3 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Gene Symbol MMP1
Gene Full Name matrix metallopeptidase 1
Background Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. This gene encodes a secreted enzyme which breaks down the interstitial collagens, types I, II, and III. The gene is part of a cluster of MMP genes which localize to chromosome 11q22.3. Alternative splicing results in multiple transcript variants.[provided by RefSeq, Mar 2009]
Function Cleaves collagens of types I, II, and III at one site in the helical domain. Also cleaves collagens of types VII and X. In case of HIV infection, interacts and cleaves the secreted viral Tat protein, leading to a decrease in neuronal Tat's mediated neurotoxicity. [UniProt]
Cellular Localization Secreted, extracellular space, extracellular matrix. [UniProt]
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PTM Undergoes autolytic cleavage to two major forms (22 kDa and 27 kDa). A minor form (25 kDa) is the glycosylated form of the 22 kDa form. The 27 kDa form has no activity while the 22/25 kDa form can act as activator for collagenase.

Tyrosine phosphorylated in platelets by PKDCC/VLK. [UniProt]
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